João Ramalho-Santos

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João Ramalho-Santos is Full Professor of Cell, Development and Reproductive Biology at the Department of Life Sciences, University of Coimbra. He is a researcher at CIBB, where he leads the Biology of Reproduction and Stem Cell Group, carrying out research in metabolism in the context of both reproduction (male and female gamete function, assisted reproduction and fertility preservation) a pluripotency. His research interests also include science communication and monitoring its impact, co-creation of materials, narrative medicine and bioethics. He has over 145 publications in ISI Journals (6300 citations; h-index 39 in Scopus), including Nature and Nature Medicine. He is evaluator for over 10 international agencies (such as FCT, NWO, FWO, DFG, CINECA, UEFISCDI and MRC), and for over 50 ISI journals. He is Associate Editor of “Reproduction”, “International Journal of Molecular Sciences”, “Fertility and Sterility- Science”, and “Biomolecules”. He has mentored 22 PhD and 34 M.Sc. students, and his current grant support is 1.34 M Euros. He authored several works of fiction (in “Nature” and, and over 20 comics on several topics related to science communication.


Department of Life Sciences, University of Coimbra


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Information about journal articles, updated at 19-03-2023, from platform CIÊNCIAVITAE.

Mitochondria-Targeted Compounds to Assess and Improve Human Sperm Function

Sara Escada-Rebelo; Maria Inês Cristo; João Ramalho-Santos; Sandra Amaral, Antioxidants & Redox Signaling. 2022. . 10.1089/ars.2021.0238 . Antioxidants & Redox Signaling

Translating Biochemistry Concepts into Cartoons and Graphic Narratives: Potential and Pitfalls

Mireia Alemany i Pagès; Rui Tavares; Anabela Marisa Azul; Joao Ramalho-Santos, BioChem. 2022. . 10.3390/biochem2010008 . BioChem

Differential Oxygen Exposure Modulates Mesenchymal Stem Cell Metabolism and Proliferation through mTOR Signaling

Inês Moniz; João Ramalho-Santos; Ana F. Branco, International Journal of Molecular Sciences. 3749 - 3749. 7. 23. 2022. . 10.3390/ijms23073749 . International Journal of Molecular Sciences

Mushrooms on the plate: Trends towards NAFLD treatment, health improvement and sustainable diets

Adriana Fontes; João Ramalho-Santos; Hans Zischka; Anabela Marisa Azul, European Journal of Clinical Investigation. 2022. . 10.1111/eci.13667 . European Journal of Clinical Investigation

The use of comics to promote health awareness: A template using nonalcoholic fatty liver disease

Mireia Alemany-Pagès; Anabela Marisa Azul; João Ramalho-Santos, European Journal of Clinical Investigation. 2022. . 10.1111/eci.13642 . European Journal of Clinical Investigation

Unhealthy lifestyles, environment, well-being and health capability in rural neighbourhoods: a community-based cross-sectional study

Azul, A.M.; Almendra, R.; Quatorze, M.; Loureiro, A.; Reis, F.; Tavares, R.; Mota-Pinto, A.; et al, BMC public health. 1628 - 1628. 1. 21. 2021. . 10.1186/s12889-021-11661-4 . BMC public health

Does supplementation with mitochondria improve oocyte competence? A systematic review

Ferreira, A.F.; Soares, M.; Reis, S.A.; Ramalho-Santos, J.; Sousa, A.P.; Almeida-Santos, T., Reproduction. 269 - 287. 3. 161. 2021. . 10.1530/REP-20-0351 . Reproduction

The impact of antisperm antibodies on human male reproductive function: An update

Silva, A.F.; Ramalho-Santos, J.; Amaral, S., Reproduction. R55 - R71. 4. 162. 2021. . 10.1530/REP-21-0123 . Reproduction

The role of sperm and oocyte in fetal programming: Is Lamarck making a comeback?

Tavares, R.S.; Ramalho-Santos, J., European Journal of Clinical Investigation. 10. 51. 2021. . 10.1111/eci.13521 . European Journal of Clinical Investigation

Comparative in vitro study on the local tolerance and efficacy of benzalkonium chloride, myristalkonium chloride and nonoxynol-9 as active principles in vaginal contraceptives

Maria Inês Alfaiate; Rita António Santos; Andreia Filipa Silva; Ana Paula Sousa; Teresa Almeida-Santos; Célia Gendron; Violaine Jabbour; et al, The European Journal of Contraception & Reproductive Health Care. 334 - 342. 4. 26. 2021. . 10.1080/13625187.2021.1900563 . The European Journal of Contraception & Reproductive Health Care

Using Data Mining to Assist in Predicting Reproductive Outcomes Following Varicocele Embolization

Ana Paula Sousa; Judith Santos-Pereira; Maria José Freire; Belmiro Parada; Teresa Almeida-Santos; Jorge Bernardino; João Ramalho-Santos, Journal of Clinical Medicine. 3503 - 3503. 16. 10. 2021. . 10.3390/jcm10163503 . Journal of Clinical Medicine

Fluorescent probes for the detection of reactive oxygen species in human spermatozoa

Escada-Rebelo, S.; Mora, F.; Sousa, A.; Almeida-Santos, T.; Paiva, A.; Ramalho-Santos, J., Asian Journal of Andrology. 465 - 471. 5. 22. 2020. . 10.4103/aja.aja_132_19 . Asian Journal of Andrology

The mTOR pathway in reproduction: from gonadal function to developmental coordination

Correia, B.; Sousa, M.I.; Ramalho-Santos, J., Reproduction (Cambridge, England). R173 - R188. 4. 159. 2020. . 10.1530/REP-19-0057 . Reproduction (Cambridge, England)

Aging-related mitochondrial alterations in bovine oocytes

Soares, M.; Sousa, A.P.; Fernandes, R.; Ferreira, A.F.; Almeida-Santos, T.; Ramalho-Santos, J., Theriogenology. 218 - 225. 157. 2020. . 10.1016/j.theriogenology.2020.07.036 . Theriogenology

Insights from qualitative research on NAFLD awareness with a cohort of T2DM patients: Time to go public with insulin resistance?

Alemany-Pagès, M.; Moura-Ramos, M.; Araújo, S.; MacEdo, M.P.; Ribeiro, R.T.; Do Ó, D.; Ramalho-Santos, J.; Azul, A.M., BMC Public Health. 1. 20. 2020. . 10.1186/s12889-020-09249-5 . BMC Public Health

Publicly stressing the role of mitochondria in NAFLD with(in) a sports event

Mireia Alemany-Pagès; Rui Tavares; Sara Varela Amaral; Ana Teresa Barros-Viegas; Paulo J. Oliveira; João Ramalho-Santos; Anabela Marisa Azul, European Journal of Clinical Investigation. 2020. . 10.1111/eci.13234 . European Journal of Clinical Investigation

Metabolic characterization of a paused-like pluripotent state

Sousa, M.I.; Correia, B.; Rodrigues, A.S.; Ramalho-Santos, J., Biochimica et Biophysica Acta - General Subjects. 8. 1864. 2020. . 10.1016/j.bbagen.2020.129612 . Biochimica et Biophysica Acta - General Subjects

Effects of DMSO on the Pluripotency of Cultured Mouse Embryonic Stem Cells (mESCs)

Maria Inês Sousa; Bibiana Correia; Ana Filipa Branco; Ana Sofia Rodrigues; João Ramalho-Santos; Katia Mareschi, Stem Cells International. 2020. . 10.1155/2020/8835353 . Stem Cells International

Stem metabolism: Insights from oncometabolism and vice versa

Rodrigues, A.S.; Pereira, S.L.; Ramalho-Santos, J., Biochimica et Biophysica Acta - Molecular Basis of Disease. 7. 1866. 2020. . 10.1016/j.bbadis.2020.165760 . Biochimica et Biophysica Acta - Molecular Basis of Disease

Sperm selection in assisted reproduction: A review of established methods and cutting-edge possibilities

Marzano, G.; Chiriacò, M.S.; Primiceri, E.; Dell'Aquila, M.E.; Ramalho-Santos, J.; Zara, V.; Ferramosca, A.; Maruccio, G., Biotechnology Advances. 2019. . 10.1016/j.biotechadv.2019.107498 . Biotechnology Advances

Can antidiabetic drugs improve male reproductive (Dys)function associated with diabetes?

Tavares, R.S.; Escada-Rebelo, S.; Sousa, M.I.; Silva, A.; Ramalho-Santos, J.; Amaral, S., Current Medicinal Chemistry. 4191 - 4222. 22. 26. 2019. . 10.2174/0929867325666181101111404 . Current Medicinal Chemistry

Antioxidant Versus Pro-Apoptotic Effects of Mushroom-Enriched Diets on Mitochondria in Liver Disease

Adriana Fontes; Mireia Alemany-Pagès; Paulo J. Oliveira; João Ramalho-Santos; Hans Zischka; Anabela Marisa Azul, International Journal of Molecular Sciences. 3987 - 3987. 16. 20. 2019. . 10.3390/ijms20163987 . International Journal of Molecular Sciences

Can we induce spermatogenesis in the domestic cat using an in vitro tissue culture approach?

Silva, A.F.; Escada-Rebelo, S.; Amaral, S.; Tavares, R.S.; Schlatt, S.; Ramalho-Santos, J.; Mota, P.C., PLoS ONE. 2. 13. 2018. . 10.1371/journal.pone.0191912 . PLoS ONE

Metabolic and Mechanical Cues Regulating Pluripotent Stem Cell Fate

Perestrelo, T.; Correia, M.; Ramalho-Santos, J.; Wirtz, D., Trends in Cell Biology. 1014 - 1029. 12. 28. 2018. . 10.1016/j.tcb.2018.09.005 . Trends in Cell Biology


Ramalho-Santos, J., Nature. 408 - 408. 7696. 555. 2018. . 10.1038/d41586-018-03054-2 . Nature

Antidiabetic therapies and male reproductive function: where do we stand?

Tavares, R.S.; Escada-Rebelo, S.; Silva, A.F.; Sousa, M.I.; Ramalho-Santos, J.; Amaral, S., Reproduction. R13 - R37. 1. 155. 2018. . 10.1530/REP-17-0390 . Reproduction

Alzheimer’s disease-related amyloid-ß<inf>1–42</inf> peptide induces the loss of human sperm function

Tavares, R.S.; Martins, S.; Almeida-Santos, T.; Sousa, A.P.; Ramalho-Santos, J.; da Cruz E Silva, O.A., Cell and Tissue Research. 1 - 5. 2017. . 10.1007/s00441-017-2665-1 . Cell and Tissue Research

Pluri-IQ: Quantification of Embryonic Stem Cell Pluripotency through an Image-Based Analysis Software

Perestrelo, T.; Chen, W.; Correia, M.; Le, C.; Pereira, S.; Rodrigues, A.S.; Sousa, M.I.; Ramalho-Santos, J.; Wirtz, D., Stem Cell Reports. 697 - 709. 2. 9. 2017. . 10.1016/j.stemcr.2017.06.006 . Stem Cell Reports

High glucose levels affect spermatogenesis: An in vitro approach

Tavares, R.S.; Portela, J.M.D.; Sousa, M.I.; Mota, P.C.; Ramalho-Santos, J.; Amaral, S., Reproduction, Fertility and Development. 1369 - 1378. 7. 29. 2017. . 10.1071/RD15475 . Reproduction, Fertility and Development

Sirtuins in metabolism, stemness and differentiation

Correia, M.; Perestrelo, T.; Rodrigues, A.S.; Ribeiro, M.F.; Pereira, S.L.; Sousa, M.I.; Ramalho-Santos, J., Biochimica et Biophysica Acta - General Subjects. 3444 - 3455. 1. 1861. 2017. . 10.1016/j.bbagen.2016.09.008 . Biochimica et Biophysica Acta - General Subjects

Different concentrations of kaempferol distinctly modulate murine embryonic stem cell function

Marcelo Correia; Ana S. Rodrigues; Tânia Perestrelo; Sandro L. Pereira; Marcelo F. Ribeiro; Maria I. Sousa; João Ramalho-Santos, Food and Chemical Toxicology. 148 - 156. 87. 2016. . 10.1016/j.fct.2015.12.011 . Food and Chemical Toxicology

The non-genomic effects of endocrine-disrupting chemicals on mammalian sperm

Tavares, R.S.; Escada-Rebelo, S.; Correia, M.; Mota, P.C.; Ramalho-Santos, J., Reproduction. R1 - R13. 1. 151. 2016. . 10.1530/REP-15-0355 . Reproduction

Cytotoxic potential of decidual NK cells and CD8+ T cells awakened by infections

Crespo, Â.C.; van der Zwan, A.; Ramalho-Santos, J.; Strominger, J.L.; Tilburgs, T., Journal of Reproductive Immunology. 2016. . 10.1016/j.jri.2016.08.001 . Journal of Reproductive Immunology

Mitochondrial functionality and chemical compound action on sperm function

Amaral, S.; Tavares, R.S.; Baptista, M.; Sousa, M.I.; Silva, A.; Escada-Rebelo, S.; Paiva, C.P.; Ramalho-Santos, J., Current Medicinal Chemistry. 3575 - 3606. 31. 23. 2016. . 10.2174/0929867323666160425113518 . Current Medicinal Chemistry

Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin

Escada-Rebelo, S.; Silva, A.F.; Amaral, S.; Tavares, R.S.; Paiva, C.; Schlatt, S.; Ramalho-Santos, J.; Mota, P.C., Andrology. 2016. . 10.1111/andr.12223 . Andrology

Data on the potential impact of food supplements on the growth of mouse embryonic stem cells

Correia, M.; Sousa, M.I.; Rodrigues, A.S.; Perestrelo, T.; Pereira, S.L.; Ribeiro, M.F.; Ramalho-Santos, J., Data in Brief. 1190 - 1195. 7. 2016. . 10.1016/j.dib.2016.03.098 . Data in Brief

Mitochondrial Mechanisms of Metabolic Reprogramming in Proliferating Cells

Sousa, M.I.; Rodrigues, A.S.; Pereira, S.; Perestrelo, T.; Correia, M.; Ramalho-Santos, J., Current Medicinal Chemistry. 2493 - 2504. 20. 22. 2015. . Current Medicinal Chemistry

Dichloroacetate, the pyruvate dehydrogenase complex and the modulation of mESC pluripotency

Rodrigues, A.S.; Correia, M.; Gomes, A.; Pereira, S.L.; Perestrelo, T.; Sousa, M.I.; Ramalho-Santos, J., PLoS ONE. 7. 10. 2015. . 10.1371/journal.pone.0131663 . PLoS ONE

High glucose concentrations per se do not adversely affect human sperm function in vitro

Portela, J.M.D.; Tavares, R.S.; Mota, P.C.; Ramalho-Santos, J.; Amaral, S., Reproduction. 77 - 84. 1. 150. 2015. . 10.1530/REP-15-0100 . Reproduction

Identification of endogenous metabolites in human sperm cells using proton nuclear magnetic resonance (<sup>1</sup>H-NMR) spectroscopy and gas chromatography-mass spectrometry (GC-MS)

Paiva, C.; Amaral, A.; Rodriguez, M.; Canyellas, N.; Correig, X.; Ballescà, J.L.; Ramalho-Santos, J.; Oliva, R., Andrology. 496 - 505. 3. 3. 2015. . 10.1111/andr.12027 . Andrology

In vitro exposure to the organochlorine p,p'-DDE affects functional human sperm parameters

Tavares, R.S.; Amaral, S.; Paiva, C.; Baptista, M.; Ramalho-Santos, J., Chemosphere. 443 - 446. 120. 2015. . 10.1016/j.chemosphere.2014.08.075 . Chemosphere

Differentiate or die: 3-Bromopyruvate and pluripotency in mouse embryonic stem cells

Rodrigues, A.S.; Pereira, S.L.; Correia, M.; Gomes, A.; Perestrelo, T.; Ramalho-Santos, J., PLoS ONE. 8. 10. 2015. . 10.1371/journal.pone.0135617 . PLoS ONE

Geography of follicle formation in the embryonic mouse ovary impacts activation pattern during the first wave of folliculogenesis

Cordeiro, M.H.; Kim, S.-Y.; Ebbert, K.; Duncan, F.E.; Ramalho-Santos, J.; Woodruff, T.K., Biology of Reproduction. 4. 93. 2015. . 10.1095/biolreprod.115.131227 . Biology of Reproduction

I want more and better cells! - An outreach project about stem cells and its impact on the general population

Amaral, S.V.; Forte, T.; Ramalho-Santos, J.; Da Cruz, M.T.G., PLoS ONE. 7. 10. 2015. . 10.1371/journal.pone.0133753 . PLoS ONE

Nucleolin overexpression in breast cancer cell sub-populations with different stem-like phenotype enables targeted intracellular delivery of synergistic drug combination

Fonseca, N.A.; Rodrigues, A.S.; Rodrigues-Santos, P.; Alves, V.; Gregório, A.C.; Valério-Fernandes, Â.; Gomes-da-Silva, L.C.; et al, Biomaterials. 76 - 88. 69. 2015. . 10.1016/j.biomaterials.2015.08.007 . Biomaterials

From gametogenesis and stem cells to cancer: Common metabolic themes

Pereira, S.L.; Rodrigues, A.S.; Sousa, M.I.; Correia, M.; Perestrelo, T.; Ramalho-Santos, J., Human Reproduction Update. 924 - 943. 6. 20. 2014. . 10.1093/humupd/dmu034 . Human Reproduction Update

Concentration-dependent Sildenafil citrate (Viagra) effects on ROS production, energy status, and human sperm function

Sousa, M.I.; Amaral, S.; Tavares, R.S.; Paiva, C.; Ramalho-Santos, J., Systems Biology in Reproductive Medicine. 72 - 79. 2. 60. 2014. . 10.3109/19396368.2013.867380 . Systems Biology in Reproductive Medicine


Ramalho-Santos, J., Nature. 7505. 510. 2014. . 10.1038/510436a . Nature

Low amounts of mitochondrial reactive oxygen species define human sperm quality

Marques, M.; Sousa, A.P.; Paiva, A.; Almeida-Santos, T.; Ramalho-Santos, J., Reproduction. 817 - 824. 6. 147. 2014. . 10.1530/REP-13-0644 . Reproduction

Identification of proteins involved in human sperm motility using high-throughput differential proteomics

Amaral, A.; Paiva, C.; Attardo Parrinello, C.; Estanyol, J.M.; Ballescà, J.L.; Ramalho-Santos, J.; Oliva, R., Journal of Proteome Research. 5670 - 5684. 12. 13. 2014. . 10.1021/pr500652y . Journal of Proteome Research

Sins of the fathers: Sperm DNA damage in the context of assisted reproduction

Ramalho-Santos, J., Human Reproduction. 2356 - 2358. 11. 29. 2014. . 10.1093/humrep/deu229 . Human Reproduction

The combined human sperm proteome: Cellular pathways and implications for basic and clinical science

Amaral, A.; Castillo, J.; Ramalho-Santos, J.; Oliva, R., Human Reproduction Update. 40 - 62. 1. 20. 2014. . 10.1093/humupd/dmt046 . Human Reproduction Update

Relation of cumulus cell status with single oocyte maturity, fertilization capability and patient age

Lourenço, B.; Sousa, A.P.; Almeida-Santos, T.; Ramalho-Santos, J., Journal of Reproduction and Infertility. 15 - 21. 1. 15. 2014. . Journal of Reproduction and Infertility

Spermicidal and microbicidal compounds: In search of an efficient multipurpose strategy

Baptista, M.; Tavares, R.; Ramalho-Santos, J., Current Medicinal Chemistry. 3693 - 3700. 32. 21. 2014. . Current Medicinal Chemistry

Mitochondria and mammalian reproduction

Ramalho-Santos, J.; Amaral, S., Molecular and Cellular Endocrinology. 74 - 84. 1-2. 379. 2013. . 10.1016/j.mce.2013.06.005 . Molecular and Cellular Endocrinology

Ca2+ signals generated by CatSper and Ca2+ stores regulate different behaviors in human sperm

Alasmari, W.; Costello, S.; Correia, J.; Oxenham, S.K.; Morris, J.; Fernandes, L.; Ramalho-Santos, J.; et al, Journal of Biological Chemistry. 6248 - 6258. 9. 288. 2013. . 10.1074/jbc.M112.439356 . Journal of Biological Chemistry

Anterior positioning of sex chromosomes on the head of human sperm sorted using visible wavelengths

Alçada-Morais, S.; Sousa, A.P.; Paiva, A.; Almeida-Santos, T.; Ramalho-Santos, J., Systems Biology in Reproductive Medicine. 223 - 226. 4. 59. 2013. . 10.3109/19396368.2013.787129 . Systems Biology in Reproductive Medicine

Evaluation of human sperm chromatin status after selection using a modified Diff-Quik stain indicates embryo quality and pregnancy outcomes following in vitro fertilization

Tavares, R.S.; Silva, A.F.; Lourenço, B.; Almeida-Santos, T.; Sousa, A.P.; Ramalho-Santos, J., Andrology. 830 - 837. 6. 1. 2013. . 10.1111/j.2047-2927.2013.00127.x . Andrology

Dioxin-induced acute cardiac mitochondrial oxidative damage and increased activity of ATP-sensitive potassium channels in Wistar rats

Pereira, S.P.; Pereira, G.C.; Pereira, C.V.; Carvalho, F.S.; Cordeiro, M.H.; Mota, P.C.; Ramalho-Santos, J.; Moreno, A.J.; Oliveira, P.J., Environmental Pollution. 281 - 290. 180. 2013. . 10.1016/j.envpol.2013.05.049 . Environmental Pollution

Human sperm tail proteome suggests new endogenous metabolic pathways

Amaral, A.; Castillo, J.; Estanyol, J.M.; Ballesca, J.L.; Ramalho-Santos, J.; Oliva, R., Molecular and Cellular Proteomics. 330 - 342. 2. 12. 2013. . 10.1074/mcp.M112.020552 . Molecular and Cellular Proteomics

How can ethics relate to science? The case of stem cell research.

Carvalho, A.S.; Ramalho-Santos, J., European journal of human genetics : EJHG. 591 - 595. 6. 21. 2013. . 10.1038/ejhg.2012.232 . European journal of human genetics : EJHG

Inhibition of mitochondrial complex III blocks neuronal differentiation and maintains embryonic stem cell pluripotency

Pereira, S.L.; Grãos, M.; Rodrigues, A.S.; Anjo, S.I.; Carvalho, R.A.; Oliveira, P.J.; Arenas, E.; Ramalho-Santos, J., PLoS ONE. 12. 8. 2013. . 10.1371/journal.pone.0082095 . PLoS ONE

In vitro effects of cationic compounds on functional human sperm parameters

Baptista, M.; Publicover, S.J.; Ramalho-Santos, J., Fertility and Sterility. 705 - 712. 3. 99. 2013. . 10.1016/j.fertnstert.2012.11.008 . Fertility and Sterility

Aging and male reproductive function: A mitochondrial perspective

Amaral, S.; Amaral, A.; Ramalho-Santos, J., Frontiers in Bioscience - Scholar. 181 - 197. 1. 5 S. 2013. . Frontiers in Bioscience - Scholar

Mitochondria functionality and sperm quality

Amaral, A.; Lourenço, B.; Marques, M.; Ramalho-Santos, J., Reproduction. 5. 146. 2013. . 10.1530/REP-13-0178 . Reproduction

UVB irradiation as a tool to assess ROS-induced damage in human spermatozoa

Amaral, S.; Redmann, K.; Sanchez, V.; Mallidis, C.; Ramalho-Santos, J.; Schlatt, S., Andrology. 707 - 714. 5. 1. 2013. . 10.1111/j.2047-2927.2013.00098.x . Andrology

The male gamete is not a somatic cell-the possible meaning of varying sperm RNA levels

Amaral, A.; Ramalho-Santos, J., Antioxidants and Redox Signaling. 179 - 180. 2. 18. 2013. . 10.1089/ars.2012.4715 . Antioxidants and Redox Signaling

p,p'-DDE activates CatSper and compromises human sperm function at environmentally relevant concentrations.

Tavares, R.S.; Mansell, S.; Barratt, C.L.; Wilson, S.M.; Publicover, S.J.; Ramalho-Santos, J., Human reproduction (Oxford, England). 3167 - 3177. 12. 28. 2013. . Human reproduction (Oxford, England)

Acute effects of TCDD administration: special emphasis on testicular and sperm mitochondrial function

RamalhoSantos, João; Mota, P.C.; Tavares, R.S.; Cordeiro, M.; Pereira, S.P.; Publicover, S.J.; Oliveira, P.J.; Ramalho-Santos, J., Asian Pacific Journal of Reproduction. 269 - 276. 4. 1. 2012. . 10.1016/s2305-0500(13)60091-3 . Asian Pacific Journal of Reproduction


Ramalho-Santos, J., Nature. 7391. 483. 2012. . 10.1038/483642a . Nature

Effects of different storage protocols on cat testis tissue potential for xenografting and recovery of spermatogenesis

Mota, P.C.; Ehmcke, J.; Westernströer, B.; Gassei, K.; Ramalho-Santos, J.; Schlatt, S., Theriogenology. 299 - 310. 2. 77. 2012. . 10.1016/j.theriogenology.2011.07.042 . Theriogenology

A sperms tail: The importance of getting it right

Ramalho-Santos, J., Human Reproduction. 2590 - 2591. 9. 26. 2011. . 10.1093/humrep/der200 . Human Reproduction


Ramalho-Santos, J., Nature. 7352. 474. 2011. . 10.1038/474536a . Nature

Exogenous glucose improves long-standing human sperm motility, viability, and mitochondrial function

Amaral, A.; Paiva, C.; Baptista, M.; Sousa, A.P.; Ramalho-Santos, J., Fertility and Sterility. 848 - 850. 4. 96. 2011. . 10.1016/j.fertnstert.2011.07.1091 . Fertility and Sterility

Human procreation in unchartered territory: New twists in ethical discussions

Ramalho-Santos, J., Human Reproduction. 1284 - 1287. 6. 26. 2011. . 10.1093/humrep/der093 . Human Reproduction

Differential effects of p,p'-DDE on testis and liver mitochondria:Implications for reproductive toxicology

Mota, P.C.; Cordeiro, M.; Pereira, S.P.; Oliveira, P.J.; Moreno, A.J.; Ramalho-Santos, J., Reproductive Toxicology. 80 - 85. 1. 31. 2011. . 10.1016/j.reprotox.2010.09.010 . Reproductive Toxicology

Energy metabolism in human pluripotent stem cells and their differentiated counterparts

Varum, S.; Rodrigues, A.S.; Moura, M.B.; Momcilovic, O.; Easley IV, C.A.; Ramalho-Santos, J.; van Houten, B.; Schatten, G., PLoS ONE. 6. 6. 2011. . 10.1371/journal.pone.0020914 . PLoS ONE

Not all sperm are equal: Functional mitochondria characterize a subpopulation of human sperm with better fertilization potential

Sousa, A.P.; Amaral, A.; Baptista, M.; Tavares, R.; Campo, P.C.; Peregrín, P.C.; Freitas, A.; et al, PLoS ONE. 3. 6. 2011. . 10.1371/journal.pone.0018112 . PLoS ONE

Xenografting as a Tool to Preserve Endangered Species: Outcomes and Challenges in Model Systems

Paula C. Mota; João Ramalho-Santos; Stefan Schlatt, Veterinary Medicine International. 1 - 7. 2011. 2011. . 10.4061/2011/629409 . Veterinary Medicine International

In vitro surfactant structure-toxicity relationships: Implications for surfactant use in sexually transmitted infection prophylaxis and contraception

Inácio, A.S.; Mesquita, K.A.; Baptista, M.; Ramalho-Santos, J.; Vaz, W.L.C.; Vieira, O.V., PLoS ONE. 5. 6. 2011. . 10.1371/journal.pone.0019850 . PLoS ONE

Metabolic remodeling during H9c2 myoblast differentiation: Relevance for in vitro toxicity studies

Pereira, S.L.; Ramalho-Santos, J.; Branco, A.F.; Sardão, V.A.; Oliveira, P.J.; Carvalho, R.A., Cardiovascular Toxicology. 180 - 190. 2. 11. 2011. . 10.1007/s12012-011-9112-4 . Cardiovascular Toxicology

Assessment of mitochondrial potential: Implications for the correct monitoring of human sperm function

Amaral, A.; Ramalho-Santos, J., International Journal of Andrology. 1. 33. 2010. . 10.1111/j.1365-2605.2009.00987.x . International Journal of Andrology

Seasonal functional relevance of sperm characteristics in equine spermatozoa

Gamboa, S.; Rodrigues, A.S.; Henriques, L.; Batista, C.; Ramalho-Santos, J., Theriogenology. 950 - 958. 7. 73. 2010. . 10.1016/j.theriogenology.2009.11.023 . Theriogenology

Proton leak modulation in testicular mitochondria affects reactive oxygen species production and lipid peroxidation

Rodrigues, A.S.; Lacerda, B.; Moreno, A.J.M.; Ramalho-Santos, J., Cell Biochemistry and Function. 224 - 231. 3. 28. 2010. . 10.1002/cbf.1644 . Cell Biochemistry and Function

Mitochondrial functionality in reproduction: From gonads and gametes to embryos and embryonic stem cells

Ramalho-Santos, J.; Varum, S.; Amaral, S.; Mota, P.C.; Sousa, A.P.; Amaral, A., Human Reproduction Update. 553 - 572. 5. 15. 2009. . 10.1093/humupd/dmp016 . Human Reproduction Update

Mitochondrial bioenergetics of testicular cells from the domestic cat (Felis catus)-A model for endangered species

Mota, P.; Amaral, S.; Martins, L.; de Lourdes Pereira, M.; Oliveira, P.J.; Ramalho-Santos, J., Reproductive Toxicology. 111 - 116. 2. 27. 2009. . 10.1016/j.reprotox.2009.01.008 . Reproductive Toxicology

Aging, mitochondria and male reproductive function.

Amaral S; Ramalho-Santos J; Amaral, S.; Ramalho-Santos, J., Current Aging Science. 165 - 173. 3. 2. 2009. . 10.2174/1874609810902030165 . Current Aging Science


Ramalho-Santos, J., Nature. 7239. 458. 2009. . 10.1038/458796a . Nature

Chd1 regulates open chromatin and pluripotency of embryonic stem cells

Gaspar-Maia, A.; Alajem, A.; Polesso, F.; Sridharan, R.; Mason, M.J.; Heidersbach, A.; Ramalho-Santos, J.; et al, Nature. 863 - 868. 7257. 460. 2009. . 10.1038/nature08212 . Nature

Dual use of Diff-Quik-like stains for the simultaneous evaluation of human sperm morphology and chromatin status

Sousa, A.P.M.; Tavares, R.S.; Velez De La Calle, J.F.; Figueiredo, H.; Almeida, V.; Almeida-Santos, T.; Ramalho-Santos, J., Human Reproduction. 28 - 36. 1. 24. 2009. . 10.1093/humrep/den365 . Human Reproduction

Testicular mitochondrial alterations in untreated streptozotocin-induced diabetic rats

Amaral, S.; Mota, P.C.; Lacerda, B.; Alves, M.; Pereira, M.d.L.; Oliveira, P.J.; Ramalho-Santos, J., Mitochondrion. 41 - 50. 1. 9. 2009. . 10.1016/j.mito.2008.11.005 . Mitochondrion

Seminal traits, suitability for semen preservation and fertility in the native Portuguese horse breeds Puro Sangue Lusitano and Sorraia: Implications for stallion classification and assisted reproduction

Gamboa, S.; Machado-Faria, M.; Ramalho-Santos, J., Animal Reproduction Science. 102 - 113. 1-4. 113. 2009. . 10.1016/j.anireprosci.2008.06.004 . Animal Reproduction Science

Spermicides, microbicides and antiviral agents: Recent advances in the development of novel multi-functional compounds

Baptista, M.; Ramalho-Santos, J., Mini-Reviews in Medicinal Chemistry. 1556 - 1567. 13. 9. 2009. . 10.2174/138955709790361548 . Mini-Reviews in Medicinal Chemistry

Parabens in male infertility-Is there a mitochondrial connection?

Tavares, R.S.; Martins, F.C.; Oliveira, P.J.; Ramalho-Santos, J.; Peixoto, F.P., Reproductive Toxicology. 1 - 7. 1. 27. 2009. . 10.1016/j.reprotox.2008.10.002 . Reproductive Toxicology

Enhancement of human embryonic stem cell pluripotency through inhibition of the mitochondrial respiratory chain

Varum, S.; Momcilovic, O.; Castro, C.; Ben-Yehudah, A.; Ramalho-Santos, J.; Navara, C.S., Stem Cell Research. 142 - 156. 2-3. 3. 2009. . 10.1016/j.scr.2009.07.002 . Stem Cell Research

Diabetes and the impairment of reproductive function: Possible role of mitochondria and reactive oxygen species

Amaral, S.; Oliveira, P.J.; Ramalho-Santos, J., Current Diabetes Reviews. 46 - 54. 1. 4. 2008. . 10.2174/157339908783502398 . Current Diabetes Reviews

Freeze-dried primate sperm retains early reproductive potential after intracytoplasmic sperm injection

Sánchez-Partida, L.G.; Simerly, C.R.; Ramalho-Santos, J., Fertility and Sterility. 742 - 745. 3. 89. 2008. . 10.1016/j.fertnstert.2007.02.066 . Fertility and Sterility

The quantification of lipid and protein oxidation in stallion spermatozoa and seminal plasma: Seasonal distinctions and correlations with DNA strand breaks, classical seminal parameters and stallion fertility

Morte, M.I.; Rodrigues, A.M.; Soares, D.; Rodrigues, A.S.; Gamboa, S.; Ramalho-Santos, J., Animal Reproduction Science. 36 - 47. 1-2. 106. 2008. . 10.1016/j.anireprosci.2007.03.020 . Animal Reproduction Science

Adição de piruvato de sódio e trolox ao diluidor utilizado para congelação de sêmen de garanhões férteis e subférteis

RamalhoSantos, João, 2008. 10.1590/s0103-84782008000800028 .

Testicular aging involves mitochondrial dysfunction as well as an increase in UCP2 levels and proton leak

Amaral, S.; Mota, P.; Rodrigues, A.S.; Martins, L.; Oliveira, P.J.; Ramalho-Santos, J., FEBS Letters. 4191 - 4196. 30. 582. 2008. . 10.1016/j.febslet.2008.11.020 . FEBS Letters

Surfactants as microbicides and contraceptive agents: A systematic In Vitro study

Vieira, O.V.; Hartmann, D.O.; Cardoso, C.M.P.; Oberdoerfer, D.; Baptista, M.; Santos, M.A.S.; Almeida, L.; Ramalho-Santos, J.; Vaz, W.L.C., PLoS ONE. 8. 3. 2008. . 10.1371/journal.pone.0002913 . PLoS ONE

Characterization of human sperm populations using conventional parameters, surface ubiquitination, and apoptotic markers

Varum, S.; Bento, C.; Sousa, A.P.M.; Gomes-Santos, C.S.S.; Henriques, P.; Almeida-Santos, T.; Teodósio, C.; Paiva, A.; Ramalho-Santos, J., Fertility and Sterility. 572 - 583. 3. 87. 2007. . 10.1016/j.fertnstert.2006.07.1528 . Fertility and Sterility

The expression of polymerase gamma and mitochondrial transcription factor A and the regulation of mitochondrial DNA content in mature human sperm

Amaral, A.; Ramalho-Santos, J.; St John, J.C., Human Reproduction. 1585 - 1596. 6. 22. 2007. . 10.1093/humrep/dem030 . Human Reproduction

Flow cytometry evaluation of lead and cadmium effects on mouse spermatogenesis

Oliveira, H.; Loureiro, J.; Filipe, L.; Santos, C.; Ramalho-Santos, J.; Sousa, M.; Pereira, M.d.L., Reproductive Toxicology. 529 - 535. 3. 22. 2006. . 10.1016/j.reprotox.2006.03.009 . Reproductive Toxicology

Localization of SNAREs., NSF and Caveolin 1 in human spermatozoa: Relationship with seminal parameters

Sousa, A.P.M.; Gomes-Santos, C.S.S.; Ramalho-Santos, J., Systems Biology in Reproductive Medicine. 347 - 353. 5. 52. 2006. . 10.1080/01485010600667050 . Systems Biology in Reproductive Medicine

Aberrant nucleo-cytoplasmic cross-talk results in donor cell mtDNA persistence in cloned embryos

Lloyd, R.E.; Lee, J.-H.; Alberio, R.; Bowles, E.J.; Ramalho-Santos, J.; Campbell, K.H.S.; St. John, J.C., Genetics. 2515 - 2527. 4. 172. 2006. . 10.1534/genetics.105.055145 . Genetics

Effects of hyperglycemia on sperm and testicular cells of Goto-Kakizaki and streptozotocin-treated rat models for diabetes

Amaral, S.; Moreno, A.J.; Santos, M.S.; Seiça, R.; Ramalho-Santos, J., Theriogenology. 2056 - 2067. 9. 66. 2006. . 10.1016/j.theriogenology.2006.06.006 . Theriogenology

Histopathological effects of hexavalent chromium in mouse kidney

Oliveira, H.; Santos, T.M.; Ramalho-Santos, J.; Pereira, M.D.L., Bulletin of Environmental Contamination and Toxicology. 977 - 983. 6. 76. 2006. . 10.1007/s00128-006-1014-y . Bulletin of Environmental Contamination and Toxicology

The analysis of mitochondria and mitochondrial DNA in human embryonic stem cells.

St John JC; Amaral A; Bowles E; Oliveira JF; Lloyd R; Freitas M; Gray HL; et al, Methods in molecular biology (Clifton, N.J.). 347 - 374. 331. 2006. . 10.1385/1-59745-046-4:347 . Methods in molecular biology (Clifton, N.J.)

Comparison between different markers for sperm quality in the cat: Diff-Quik as a simple optical technique to assess changes in the DNA of feline epididymal sperm

Mota, P.C.; Ramalho-Santos, J., Theriogenology. 1360 - 1375. 7. 65. 2006. . 10.1016/j.theriogenology.2005.08.016 . Theriogenology

The expression of mitochondrial DNA transcription factors during early cardiomyocyte in vitro differentiation from human embryonic stemn cells

St. John, J.C.; Ramalho-Santos, J.; Gray, H.L.; Petrosko, P.; Rawe, V.Y.; Navara, C.S.; Simerly, C.R.; Schatten, G.P., Cloning and Stem Cells. 141 - 153. 3. 7. 2005. . 10.1089/clo.2005.7.141 . Cloning and Stem Cells

SNARE proteins and caveolin-1 in stallion spermatozoa: Possible implications for fertility

Gamboa, S.; Ramalho-Santos, J., Theriogenology. 275 - 291. 2. 64. 2005. . 10.1016/j.theriogenology.2004.11.021 . Theriogenology

Effect of lead chloride on spermatogenesis and sperm parameters in mice

Graça, A.; Ramalho-Santos, J.; Pereira, M.deL., Asian Journal of Andrology. 237 - 241. 3. 6. 2004. . Asian Journal of Andrology

The role of target membrane sialic acid residues in the fusion activity of the influenza virus: the effect of two types of ganglioside on the kinetics of membrane merging.

Ramalho-Santos J; Pedroso De Lima MC; Ramalho-Santos, J.; Pedroso De Lima, M.C., Cellular and Molecular Biology Letters. 337 - 351. 2. 9. 2004. . Cellular and Molecular Biology Letters

WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis

Rawe, V.Y.; Ramalho-Santos, J.; Payne, C.; Chemes, H.E.; Schatten, G., Human Reproduction. 2594 - 2604. 11. 19. 2004. . 10.1093/humrep/deh513 . Human Reproduction

Presence of N-ethyl maleimide sensitive factor (NSF) on the acrosome of mammalian sperm

Ramalho-Santos, J.; Schatten, G., Systems Biology in Reproductive Medicine. 163 - 168. 3. 50. 2004. . 10.1080/01485010490425502 . Systems Biology in Reproductive Medicine

Simultaneous analysis of cytoskeletal patterns and chromosome positioning in human fertilization failures

Ramalho-Santos, J.; Amaral, A.; Brito, R.; Freitas, M.; Almeida Santos, T., Fertility and Sterility. 1654 - 1659. 6. 82. 2004. . 10.1016/j.fertnstert.2004.05.086 . Fertility and Sterility

Let the tub drain [2]

Ramalho-Santos, J., Scientist. 13. 17. 2003. . Scientist

Ubiquitination of prohibitin in mammalian sperm mitochondria: Possible roles in the regulation of mitochondrial inheritance and sperm quality control

Thompson, W.E.; Ramalho-Santos, J.; Sutovsky, P., Biology of Reproduction. 254 - 260. 1. 69. 2003. . 10.1095/biolreprod.102.010975 . Biology of Reproduction

LIS1 Association with Dynactin is Required for Nuclear Motility and Genomic Union in the Fertilized Mammalian Oocyte

Payne, C.; St. John, J.C.; Ramalho-Santos, J.; Schatten, G., Cell Motility and the Cytoskeleton. 245 - 251. 4. 56. 2003. . 10.1002/cm.10151 . Cell Motility and the Cytoskeleton

Preferentially localized dynein and perinuclear dynactin associate with nuclear pore complex proteins to mediate genomic union during mammalian fertilization

Payne, C.; Rawe, V.; Ramalho-Santos, J.; Simerly, C.; Schatten, G., Journal of Cell Science. 4727 - 4738. 23. 116. 2003. . 10.1242/jcs.00784 . Journal of Cell Science

Rebuttal of a role for the epididymis in sperm quality control by phagocytosis of defective sperm (multiple letters)

Cooper, T.G.; Yeung, C.-H.; Jones, R.; Orgein-Crist, M.-C.; Robaire, B.; Sutovsky, P.; Moreno, R.D.; et al, Journal of Cell Science. 5 - 7. 1. 115. 2002. . Journal of Cell Science

Golgi apparatus dynamics during mouse oocyte in vitro maturation: Effect of the membrane trafficking inhibitor brefeldin A

Moreno, R.D.; Schatten, G.; Ramalho-Santos, J., Biology of Reproduction. 1259 - 1266. 5. 66. 2002. . Biology of Reproduction

VAMP/synaptobrevin as an acrosomal marker for human sperm

Ramalho-Santos, J.; Terada, Y.; Schatten, G., Fertility and Sterility. 159 - 161. 1. 77. 2002. . 10.1016/S0015-0282(01)02957-0 . Fertility and Sterility

Fluorescent probes for monitoring virus fusion kinetics: comparative evaluation of reliability

RamalhoSantos, João, 2002. 10.1016/s0005-2736(01)00457-6 .

Fluorescent probes for monitoring virus fusion kinetics: Comparative evaluation of reliability

Nunes-Correia, I.; Eulálio, A.; Nir, S.; Düzgünes, N.; Ramalho-Santos, J.; Pedroso De Lima, M.C., Biochimica et Biophysica Acta - Biomembranes. 65 - 75. 1. 1561. 2002. . 10.1016/S0005-2736(01)00457-6 . Biochimica et Biophysica Acta - Biomembranes

Control of membrane fusion during spermiogenesis and the acrosome reaction

Ramalho-Santos, J.; Schatten, G.; Moreno, R.D., Biology of Reproduction. 1043 - 1051. 4. 67. 2002. . Biology of Reproduction

Acrosome components after intracytoplasmic sperm injection: the decondensation frontier

RamalhoSantos, João, 2001. 10.1016/s0015-0282(01)01835-0 .

Membrane trafficking machinery components associated with the mammalian acrosome during spermiogenesis

Ramalho-Santos, J.; Moreno, R.D.; Wessel, G.M.; Chan, E.K.L.; Schatten, G., Experimental Cell Research. 45 - 60. 1. 267. 2001. . 10.1006/excr.2000.5119 . Experimental Cell Research

A putative, ubiquitin-dependent mechanism for the recognition and elimination of defective spermatozoa in the mammalian epididymis

Sutovsky, P.; Moreno, R.; Ramalho-Santos, J.; Dominko, T.; Thompson, W.E.; Schatten, G., Journal of Cell Science. 1665 - 1675. 9. 114. 2001. . Journal of Cell Science

Targeting and fusion proteins during mammalian spermiogenesis

Ramalho-Santos, J.; Moreno, R.D., Biological Research. 147 - 152. 2. 34. 2001. . Biological Research

Fusion and infection of influenza and sendai viruses as modulated by dextran sulfate: A comparative study

Ramalho-Santos, J.; Pedroso De Lima, M.C., Bioscience Reports. 293 - 304. 3. 21. 2001. . 10.1023/A:1013282015121 . Bioscience Reports

Acrosome components after intracytoplasmic sperm injection: The decondensation frontier

Ramalho-Santos, J.; Simerly, C.R.; Hewitson, L.; Schatten, G., Fertility and Sterility. 196 - 197. 1. 76. 2001. . 10.1016/S0015-0282(01)01835-0 . Fertility and Sterility

Foreign DNA transmission by ICSI: Injection of spermatozoa bound with exogenous DNA results in embryonic GFP expression and live rhesus monkey births

Chan, A.W.S.; Luetjens, C.M.; Dominko, T.; Ramalho-Santos, J.; Simerly, C.R.; Hewitson, L.; Schatten, G., Molecular Human Reproduction. 26 - 33. 1. 6. 2000. . Molecular Human Reproduction

ICSI choreography: Fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications

Ramalho-Santos, J.; Sutovsky, P.; Simerly, C.; Oko, R.; Wessel, G.M.; Hewitson, L.; Schatten, G., Human Reproduction. 2610 - 2620. 12. 15. 2000. . Human Reproduction

Vesicular traffic and Golgi apparatus dynamics during mammalian spermatogenesis: Implications for acrosome architecture

Moreno, R.D.; Ramalho-Santos, J.; Sutovsky, P.; Chan, E.K.L.; Schatten, G., Biology of Reproduction. 89 - 98. 1. 63. 2000. . Biology of Reproduction

TransgenICSI reviewed: Foreign DNA transmission by intracytoplasmic sperm injection in rhesus monkey

Chan, A.W.S.; Luetjens, C.M.; Dominko, T.; Ramalho-Santos, J.; Simerly, C.R.; Hewitson, L.; Schatten, G., Molecular Reproduction and Development. 325 - 328. 2 SUPPL.. 56. 2000. . Molecular Reproduction and Development

Ubiquitinated sperm mitochondria, selective proteolysis, and the regulation of mitochondrial inheritance in mammalian embryos

Sutovsky, P.; Moreno, R.D.; Ramalho-Santos, J.; Dominko, T.; Simerly, C.; Schatten, G., Biology of Reproduction. 582 - 590. 2. 63. 2000. . Biology of Reproduction

The Golgi apparatus segregates from the lysosomal/acrosomal vesicle during rhesus spermiogenesis: Structural alterations

Moreno, R.D.; Ramalho-Santos, J.; Chan, E.K.L.; Wessel, G.M.; Schatten, G., Developmental Biology. 334 - 349. 2. 219. 2000. . 10.1006/dbio.2000.9606 . Developmental Biology

SNAREs in mammalian sperm: Possible implications for fertilization

Ramalho-Santos, J.; Moreno, R.D.; Sutovsky, P.; Chan, A.W.-S.; Hewitson, L.; Wessel, G.M.; Simerly, C.R.; Schatten, G., Developmental Biology. 54 - 69. 1. 223. 2000. . 10.1006/dbio.2000.9745 . Developmental Biology

Unique checkpoints during the first cell cycle of fertilization after intracytoplasmic sperm injection in rhesus monkeys

Hewitson, L.; Dominko, T.; Takahashi, D.; Martinovich, C.; Ramalho-Santos, J.; Sutovsky, P.; Fanton, J.; et al, Nature Medicine. 431 - 433. 4. 5. 1999. . 10.1038/7430 . Nature Medicine

Ubiquitin tag for sperm mitochondria

Sutovsky, P.; Moreno, R.D.; Ramalho-Santos, J.; Dominko, T.; Simerly, C.; Schatten, G., Nature. 371 - 372. 6760. 402. 1999. . 10.1038/46466 . Nature

On-stage selection of single round spermatids using a vital, mitochondrion-specific fluorescent probe MitoTracker(TM) and high resolution differential interference contrast microscopy

Sutovsky, P.; Ramalho-Santos, J.; Moreno, R.D.; Oko, R.; Hewitson, L.; Schatten, G., Human Reproduction. 2301 - 2312. 9. 14. 1999. . 10.1093/humrep/14.9.2301 . Human Reproduction

Role of a transbilayer pH gradient in the membrane fusion activity of the influenza virus hemagglutinin: Use of the R18 assay to monitor membrane merging

RamalhoSantos, João; Ramalho-Santos, J.; Pedroso De Lima, M.C., Biological Procedures Online. 107 - 113. 3. 1. 1999. . 10.1251/bpo13 . Biological Procedures Online

Interactions of influenza virus with cultured cells: Detailed kinetic modeling of binding and endocytosis

Nunes-Correia, I.; Ramalho-Santos, J.; Nir, S.; Pedroso De Lima, M.C., Biochemistry. 1095 - 1101. 3. 38. 1999. . 10.1021/bi9812524 . Biochemistry

Optimization strategies for production of mammalian embryos by nuclear transfer

Dominko, T.; Ramalho-Santos, J.; Chan, A.; Moreno, R.D.; Luetjens, C.M.; Simerly, C.; Hewitson, L.; et al, Cloning and Stem Cells. 143 - 152. 3. 1. 1999. . Cloning and Stem Cells

Sendai virus fusion activity as modulated by target membrane components

Nunes-Correia, I.; Ramalho-Santos, J.; Pedroso De Lima, M.C., Bioscience Reports. 59 - 68. 2. 18. 1998. . 10.1023/A:1020180109275 . Bioscience Reports

The influenza virus hemagglutinin: A model protein in the study of membrane fusion

Ramalho-Santos, J.; De Lima, M.C.P.; Almeida, M.T.; De Pedroso Lima, M.C., Biochimica et Biophysica Acta - Reviews on Biomembranes. 147 - 154. 1. 1376. 1998. . 10.1016/S0304-4157(98)00002-1 . Biochimica et Biophysica Acta - Reviews on Biomembranes

Fusion activity of the influenza virus hemagglutinin does not require a transbilayer pH gradient

Ramalho-Santos, J.; Pedroso De Lima, M.C., Biochimica et Biophysica Acta - Biomembranes. 194 - 198. 2. 1330. 1997. . 10.1016/S0005-2736(97)00166-1 . Biochimica et Biophysica Acta - Biomembranes

Evidence that synaptobrevin is involved in fusion between synaptic vesicles and synaptic plasma membrane vesicles

Almeida, M.T.; Ramalho-Santos, J.; Oliveira, C.R.; Pedroso De Lima, M.C., Biochemical and Biophysical Research Communications. 184 - 188. 1. 236. 1997. . 10.1006/bbrc.1997.6928 . Biochemical and Biophysical Research Communications

Partial fusion activity of influenza virus toward liposomes and erythrocyte ghosts is distinct from viral inactivation

Ramalho-Santos, J.; De Pedroso Lima, M.C.; Nir, S., Journal of Biological Chemistry. 23902 - 23906. 39. 271. 1996. . 10.1074/jbc.271.39.23902 . Journal of Biological Chemistry

Fusion activity of African swine fever virus towards target membranes: Lipid dependence and effect of dehydrating agents

Bernardes, C.; Valdeira, M.L.; Ramalho-Santos, J.; De Lima, M.C.P., Biochemistry and Molecular Biology International. 481 - 488. 3. 37. 1995. . Biochemistry and Molecular Biology International

Target cell membrane sialic acid modulates both binding and fusion activity of influenza virus

Pedroso de Lima, M.C.; Ramalho-Santos, J.; Flasher, D.; Slepushkin, V.A.; Nir, S.; Düzgunes, N., BBA - Biomembranes. 323 - 330. 2. 1236. 1995. . 10.1016/0005-2736(95)00067-D . BBA - Biomembranes

Fusion of Sendai virus with model membranes as affected by dehydrating agents and pH: Correlation between viral envelope hydrophobicity and fusion activity

Ramalho-Santos, J.; Negrao, R.; Da Conceicao Pedroso de Lima, M., Biochemistry and Molecular Biology International. 1121 - 1127. 6. 32. 1994. . Biochemistry and Molecular Biology International

Role of hydrophobic interactions in the fusion activity of influenza and sendai viruses towards model membranes

Ramalho-Santos, J.; Negrão, R.; Lima, M.d.C.P.d., Bioscience Reports. 15 - 24. 1. 14. 1994. . 10.1007/BF01901634 . Bioscience Reports

Parameters affecting fusion between liposomes and synaptosomes. Role of proteins, lipid peroxidation, pH and temperature

Almeida, M.T.; Ramalho-Santos, J.; Oliveira, C.R.; Pedroso de Lima, M.C., The Journal of Membrane Biology. 217 - 222. 2. 142. 1994. . 10.1007/BF00234943 . The Journal of Membrane Biology

A common mechanism for influenza virus fusion activity and inactivation

Ramalho-Santos, J.; Nir, S.; Düzgünes, N.; De Carvalho, A.P.; De Lima, M.D.C.P., Biochemistry. 2771 - 2779. 11. 32. 1993. . 10.1021/bi00062a006 . Biochemistry

Interaction of clathrin with large unilamellar phospholipid vesicles at neutral pH. Lipid dependence and protein penetration

Seppen, J.; Ramalho-Santos, J.; de Carvalho, A.P.; ter Beest, M.; Kok, J.W.; Pedroso de Lima, M.C.; Hoekstra, D., BBA - Biomembranes. 209 - 215. 1. 1106. 1992. . 10.1016/0005-2736(92)90240-M . BBA - Biomembranes

Kinetic modeling of Sendai virus fusion with PC-12 cells. Effect of pH and temperature on fusion and viral inactivation

PEDROSO DE LIMA, Maria da Conceicao; RAMALHO-SANTOS, Joao; MARTINS, Maria de Fatima; PATO DE CARVALHO, Arselio; BAIROS, Vasco; NIR, Shlomo, European Journal of Biochemistry. 181 - 186. 1. 205. 1992. . 10.1111/j.1432-1033.1992.tb16766.x . European Journal of Biochemistry

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Funders: Fundação para a Ciência e a Tecnologia

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Funders: Comissão de Coordenação e Desenvolvimento Regional do Centro

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INFERTILY2EXPLAIN: In search of novel biomarkers for unexplained human male infertility (CO-PI).


Funders: Fundação para a Ciência e a Tecnologia

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Searching for novel biomarkers in unknown origin male infertility


Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia; Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia

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Universidade de Coimbra

Funders: Comissão de Coordenação e Desenvolvimento Regional do Centro

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Tackling CANcer STEM CELls: a challenge and an opportunity to advance in anti-cancer therapy


Universidade do Porto Instituto de Patologia e Imunologia Molecular; Instituto Nacional de Engenharia Biomédica; Fundação Calouste Gulbenkian; Universidade de Coimbra; Universidade de Coimbra Centro de Inovação em Biomedicina e Biotecnologia

Funders: Fundação para a Ciência e a Tecnologia

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Universidade de Coimbra Instituto de Investigação Interdisciplinar

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Healthy Lifestyle Innovation Quarters for Cities and Citizens (HeaLIQs4Cities).



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The role of the pyruvate dehydrogenase kinase as a metabolic switch in pluripotency: insights into cancer and stem cell biology


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Metabolic modulation as a tool to create and propagate pluripotent stem cell lines


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Metabolism as a modulator of stem cell pluripotency and differentiation


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2009/01/01 - 2009/06/30



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Implementation of a Nacional Facility for DNA Microarrays: Phase II


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Funders: Fundação para a Ciência e a Tecnologia

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Funders: Fundação para a Ciência e a Tecnologia

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