CNC

Publications

Information about journal articles, updated at 04-06-2023, from platform CIÊNCIAVITAE.

Isolation of Extracellular Vesicles from Human Follicular Fluid: Size-Exclusion Chromatography versus Ultracentrifugation

Soares, Maria; Pinto, Maria M.; Nobre, Rui Jorge; de Almeida, Luís Pereira; da Graça Rasteiro, Maria; Almeida-Santos, Teresa; Ramalho-Santos, João; Sousa, Ana Paula, Biomolecules. 2. 13. 2023. http://dx.doi.org/10.3390/biom13020278 . 10.3390/biom13020278 . Biomolecules

Extracellular vesicle-based delivery of silencing sequences for the treatment of Machado-Joseph disease/spinocerebellar ataxia type 3

Rufino-Ramos, David; Albuquerque, Patrícia R.; Leandro, Kevin; Carmona, Vitor; Martins, Inês M.; Fernandes, Rita; Henriques, Carina; et al, Molecular Therapy. 2023. http://dx.doi.org/10.1016/j.ymthe.2023.04.001 . 10.1016/j.ymthe.2023.04.001 . Molecular Therapy

A new protocol for whole-brain biodistribution analysis of AAVs by tissue clearing, light-sheet microscopy and semi-automated spatial quantification

Lopes, Miguel M.; Paysan, Jacques; Rino, José; Lopes, Sara M.; Pereira de Almeida, Luís; Cortes, Luísa; Nobre, Rui Jorge, Gene Therapy. 665 - 679. 12. 29. 2022. http://hdl.handle.net/10451/55435 . 10.1038/s41434-022-00372-z . Gene Therapy

Cerebellar morphometric and spectroscopic biomarkers for Machado-Joseph Disease

Miranda, Catarina Oliveira; Nobre, Rui Jorge; Paiva, Vítor Hugo; Duarte, João Valente; Castelhano, João; Petrella, Lorena Itati; Sereno, José; et al, Acta Neuropathologica communications . 2022. http://hdl.handle.net/10316/102828 . https://doi.org/10.1186/s40478-022-01329-4 . in press Acta Neuropathologica communications

Autophagy in Spinocerebellar ataxia type 2, a dysregulated pathway, and a target for therapy

Marcelo, Adriana; Afonso, Inês T.; Afonso-Reis, Ricardo; Brito, David V. C.; Costa, Rafael G.; Rosa, Ana; Alves-Cruzeiro, João; et al, Cell Death & Disease. 12. 12. 2021. http://dx.doi.org/10.1038/s41419-021-04404-1 . 10.1038/s41419-021-04404-1 . under revision Cell Death & Disease

MiRNA-Mediated Knockdown of ATXN3 Alleviates Molecular Disease Hallmarks in a Mouse Model for Spinocerebellar Ataxia Type 3

Nobre, Rui Jorge; Lobo, Diana D.; Henriques, Carina; Duarte, Sonia P.; Lopes, Sara M.; Silva, Ana C.; Lopes, Miguel M.; et al, Nucleic Acid Therapeutics. 2021. http://dx.doi.org/10.1089/nat.2021.0020 . 10.1089/nat.2021.0020 . Nucleic Acid Therapeutics

Highly Specific Blood-Brain Barrier Transmigrating Single-Domain Antibodies Selected by an In Vivo Phage Display Screening

Aguiar, Sandra Isabel; Dias, Joana N. R.; André, Ana Santos; Silva, Marta Lisete; Martins, Diana; Carrapiço, Belmira; Castanho, Miguel; et al, Pharmaceutics. 10. 13. 2021. http://dx.doi.org/10.3390/pharmaceutics13101598 . 10.3390/pharmaceutics13101598 . Pharmaceutics

The blood-brain barrier is disrupted in Machado-Joseph disease/spinocerebellar ataxia type 3: evidence from transgenic mice and human post-mortem samples

Nobre, Rui Jorge; Duarte Lobo, Diana; Oliveira Miranda, Catarina; Pereira, Dina; Castelhano, João; Sereno, José; Koeppen, Arnulf; Castelo-Branco, Miguel; Pereira de Almeida, Luís, Acta Neuropathologica Communications. 1. 8. 2020. http://dx.doi.org/10.1186/s40478-020-00955-0 . 10.1186/s40478-020-00955-0 . Acta Neuropathologica Communications

Trehalose alleviates the phenotype of Machado–Joseph disease mouse models

Santana, Magda M.; Paixão, Susana; Cunha-Santos, Janete; Silva, Teresa Pereira; Trevino-Garcia, Allyson; Gaspar, Laetitia S.; Nóbrega, Clévio; et al, Journal of Translational Medicine. 1. 18. 2020. http://hdl.handle.net/10400.1/13814 . 10.1186/s12967-020-02302-2 . Journal of Translational Medicine

Simple and Fast SEC-Based Protocol to Isolate Human Plasma-Derived Extracellular Vesicles for Transcriptional Research

Gaspar, Laetitia da Silva; Santana, Magda M.; Henriques, Carina; Pinto, Maria M.; Ribeiro-Rodrigues, Teresa M.; Girão, Henrique; Nobre, Rui Jorge; et al, Molecular Therapy - Methods & Clinical Development. 723 - 737. 18. 2020. http://hdl.handle.net/10316/106458 . https://doi.org/10.1016/j.omtm.2020.07.012 . Molecular Therapy - Methods & Clinical Development

Protocol for the Characterization of the CAG Tract and Flanking Polymorphisms in Machado-Joseph Disease: Impact on Diagnosis and Development of Gene-Based Therapies

Lopes, Sara Monteiro; Faro, Rosário; Lopes, Miguel Monteiro; Onofre, Isabel; Mendonça, Nuno; Ribeiro, Joana; Januário, Cristina; Nobre, Rui Jorge; Pereira de Almeida, Luís, The Journal of Molecular Diagnostics. 2020. http://dx.doi.org/10.1016/j.jmoldx.2020.03.003 . 10.1016/j.jmoldx.2020.03.003 . published The Journal of Molecular Diagnostics

Repeated Mesenchymal Stromal Cell Treatment Sustainably Alleviates Machado-Joseph Disease

Oliveira Miranda, Catarina; Marcelo, Adriana; Silva, Teresa Pereira; Barata, João; Vasconcelos-Ferreira, Ana; Pereira, Dina; Nóbrega, Clévio; et al, Molecular Therapy. 2131 - 2151. 9. 26. 2018. http://dx.doi.org/10.1016/j.ymthe.2018.07.007 . 10.1016/j.ymthe.2018.07.007 . published Molecular Therapy

Hypoxia mimetic induces lipid accumulation through mitochondrial dysfunction and stimulates autophagy in murine preadipocyte cell line

Marques, Ana Patrícia; Rosmaninho-Salgado, Joana; Estrada, Marta; Cortez, Vera; Nobre, Rui Jorge; Cavadas, Cláudia, Biochimica et Biophysica Acta (BBA) - General Subjects. 673 - 682. 3. 1861. 2017. http://dx.doi.org/10.1016/j.bbagen.2016.12.005 . 10.1016/j.bbagen.2016.12.005 . published Biochimica et Biophysica Acta (BBA) - General Subjects

Fibroblasts of Machado Joseph Disease patients reveal autophagy impairment

Onofre, Isabel; Mendonça, Nuno; Lopes, Sara; Nobre, Rui; de Melo, Joana Barbosa; Carreira, Isabel Marques; Januário, Cristina; Gonçalves, António Freire; de Almeida, Luis Pereira, Scientific Reports. 1. 6. 2016. http://dx.doi.org/10.1038/srep28220 . 10.1038/srep28220 . published Scientific Reports

Gephyrin Cleavage in In Vitro Brain Ischemia Decreases GABAA Receptor Clustering and Contributes to Neuronal Death

Costa, João T.; Mele, Miranda; Baptista, Márcio S.; Gomes, João R.; Ruscher, Karsten; Nobre, Rui J.; de Almeida, Luís Pereira; Wieloch, Tadeusz; Duarte, Carlos B., Molecular Neurobiology. 3513 - 3527. 6. 53. 2015. http://dx.doi.org/10.1007/s12035-015-9283-2 . 10.1007/s12035-015-9283-2 . published Molecular Neurobiology

Calpain inhibition reduces ataxin-3 cleavage alleviating neuropathology and motor impairments in mouse models of Machado–Joseph disease

Simões, Ana Teresa; Gonçalves, Nélio; Nobre, Rui Jorge; Duarte, Carlos Bandeira; Pereira de Almeida, Luís; Almeida, Luís Pereira de, Human Molecular Genetics. 4932 - 4944. 18. 23. 2014. http://dx.doi.org/10.1093/hmg/ddu209 . 10.1093/hmg/ddu209 . published Human Molecular Genetics

Gene Therapy for Parkinsons and Alzheimers Diseases: from the Bench to Clinical Trials

Jorge Nobre, Rui; Pereira de Almeida, Luis, Current Pharmaceutical Design. 3434 - 3445. 31. 17. 2011. http://dx.doi.org/10.2174/138161211798072472 . 10.2174/138161211798072472 . published Current Pharmaceutical Design

Characterization of common and rare human papillomaviruses in Portuguese women by the polymerase chain reaction, restriction fragment length polymorphism and sequencing

Nobre, Rui Jorge; Cruz, Eugénia; Real, Odete; de Almeida, Luís Pereira; Martins, Teresa C., Journal of Medical Virology. 1024 - 1032. 6. 82. 2010. http://dx.doi.org/10.1002/jmv.21756 . 10.1002/jmv.21756 . published Journal of Medical Virology

E7 Oncoprotein of Novel Human Papillomavirus Type 108 Lacking the E6 Gene Induces Dysplasia in Organotypic Keratinocyte Cultures

Nobre, R. J.; Herraez-Hernandez, E.; Fei, J.-W.; Langbein, L.; Kaden, S.; Grone, H.-J.; de Villiers, E.-M., Journal of Virology. 2907 - 2916. 7. 83. 2009. http://dx.doi.org/10.1128/jvi.02490-08 . 10.1128/jvi.02490-08 . published Journal of Virology

5 19 out of 19 Publications

All projects

Information of exclusive responsibility of the researcher 04-06-2023 , from platform CIÊNCIAVITAE.

2023/03/01 - 2024/08/31

Pre-clinical evaluation of the brain-targeting AAV-PHP.eB encoding cholesterol hydroxylase CYP46A1 as a therapeutic strategy for spinocerebellar ataxia type 3 – towards the understanding of the neuroprotective mechanism

2022.04788.PTDC

Universidade de Coimbra

Funders: Fundação para a Ciência e a Tecnologia

2023/03/01 - 2024/08/31

From “bedside-to-bench” biomarker discovery: on the road to a clinical trial for Machado-Joseph Disease

2022.06118.PTDC

Universidade de Coimbra

Funders: Fundação para a Ciência e a Tecnologia

2023/03/01 - 2024/08/31

Direct Conversion of Astrocytes into Cerebellar Neurons as an Innovative Treatment for Spinocerebellar Ataxia Type-3

2022.06127.PTDC

Universidade de Coimbra

Funders: Fundação para a Ciência e a Tecnologia

2022/01/01 - 2023/06/30

Genome editing to rescue Machado-Joseph disease by alternative splicing

EXPL/MED-NEU/0936/2021

Universidade de Coimbra; Universidade de Coimbra Instituto de Investigação Clínica e Biomédica de Coimbra

Funders: Fundação para a Ciência e a Tecnologia

2020/06/01 - 2020/11/30

Expedited validation and large-scale manufacture of a blowing device for collection with inactivation of samples for detection of SARS-CoV-2

269_596884125

Principal investigator

ViraVector-Unidade de Produção de vetores virais para a transferência de genes; Infogene; Universidade de Coimbra Centro de Neurociências e Biologia Celular

Funders: Fundação para a Ciência e a Tecnologia

2020/11/01 - 2025/10/31

Accelerating research & development for advanced therapies

945473

Researcher

Innovative Health Initiative

Funders: European Commission; European Federation of Pharmaceutical Industries and Associations

2019/02 - 2019/09

Targeting ATXN3 for the treatment of Spinocerebellar Ataxia 3 (SCA3) using AAV5 and an artificial microRNA

Principal investigator