Neuroscience and Disease

Redox Biology and Brain Sensing

In vivo Monitoring

Neurovascular/Neurometabolic Coupling

Micro(bio)sensor

Redox Biology

Nitrite Biology and Antioxidants


Research lines

The molecular mechanisms inherent in neuromodulation and aging under an umbrella that characterizes the bidirectional communication between neurons and microvasculature by addressing quantitatively, in vivo, and in real-time the role of nitric oxide as a diffusional intercellular messenger, coordinating the neurovascular and neurometabolic coupling axis. The study of the neurovascular-neurometabolic coupling axis, encompasses mechanistic as well nutritional approaches with potential to restore the functionality of neurovascular coupling and cognition. PI: João Laranjinha

Technological innovation in terms of the design, development and implementation of microarray technology consisting of micro(bio)sensors for the real-time monitoring of neuromodulators, neurotransmitters and metabolic intermediates in the brain of anesthetized and conscious, freely behaving animals. PI: Rui Barbosa

The mechanisms of action of plant-derived dietary phenolic compounds in terms of protection against vascular endothelial dysfunction, anti-inflammatory properties, as well as their impact on nitrite-driven regulatory processes along the nitrate:nitrite:nitric oxide pathway, encompassing the non-enzymatic production of nitric oxide from dietary nitrite in the gastric compartment and the brain. PI: Leonor Almeida

Overview

  1. To unravel the pathways which govern redox regulation of cell functions with implications for aging and neurodegeneration, namely the molecular mechanisms inherent to neurometabolic and neurovascular coupling axis, that critically involve free radicals and oxidants, in particular nitric oxide.
  2. To design and develop novel and improved micro(bio)sensors for real-time monitoring of neuromodulators, neurotransmitters and metabolic markers in brain tissue ranging from slice preparations to conscious freely-moving rodents.
  3. To understand how the nitrate:nitrite:nitric oxide pathway as affected by dietary compounds (such as polyphenols) and ensued regulatory process with impact in in gastrointestinal, cardiovascular and physiology.

 

Resources

The lab is equipped with highly specialized equipment for direct measurement of neurochemicals (nitric oxide, glutamate, ascorbate, dopamine, serotonin, etc.) and metabolic substrates/intermediates (oxygen, glucose, lactate, etc.) in vitro, ex vivo (tissue preparations, cell cultures, tissue homogenates) as well as in vivo. These include electrode and biosensors-based microarrays for brain stereotaxic insertion in vivo and chemiluminescent detector for nitric oxide in the gas phase. The lab has full-equipped setups for in vivo (anesthetized and freely moving) electrochemical recordings in the brain of rodents. Furthermore, the lab is also equipped with a chemiluminescence set up for detection of nitric oxide in the gaseous phase and nitroso and nitrated derivatives, and a high resolution respirometer (Oroboros Oxygraph 2k Instrument) which allows evaluation of oxygen consumption rates in closed chamber in preparations ranging from mitochondria and homogenates to tissue biopsies and slices.

Publications

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Information about journal articles, updated at 17-10-2021, from platform CIÊNCIAVITAE.

The Peculiar Facets of Nitric Oxide as a Cellular Messenger: From Disease-Associated Signaling to the Regulation of Brain Bioenergetics and Neurovascular Coupling

João Laranjinha; Carla Nunes; Ana Ledo; Cátia Lourenço; Bárbara Rocha; Rui M. Barbosa, 2021. Neurochemical Research. 2021. https://doi.org/10.1007/s11064-020-03015-0 . 10.1007/s11064-020-03015-0 . Neurochemical Research

The bioactivity of neuronal-derived nitric oxide in aging and neurodegeneration: switching signaling to degeneration

Laranjinha, João, 2021. Free Radical Biology & Medicine. 500 - 513. 162. 2021. published Free Radical Biology & Medicine

Nitrate from diet might fuel gut microbiota metabolism: minding the gap between redox signaling and inter-kingdom communication.

Laranjinha, João, 2020. Free Radic. Biol. Med. 37 - 43. 149. 2020. published Free Radic. Biol. Med

An optimized spectrophotometric assay reveals increased activity of enzymes involved in 2-arachidonoyl glycerol turnover in the cerebral cortex of a rat model of Alzheimer’s disease

Matilde S. Rodrigues; Cláudia Ferreira; Cândida Dias; Anna Pliássova; Lisiane Souza; Ana Ledo; João Laranjinha; Rodrigo A. Cunha; Attila Köfalvi, 2020. European Journal of Neuroscience. 2020. https://doi.org/10.1111/ejn.14944 . 10.1111/ejn.14944 . European Journal of Neuroscience

Intestinal cytotoxicity induced by Escheria coli is fully prevented by red wine polyphenol extract: mechanistic insights in epithelial cells

Laranjinha, João, 2019. Chemico-Biological Interactions. 310. 2019. published Chemico-Biological Interactions

Inorganic nitrate prevents the loss of tight junction proteins and modulates inflammatory events induced by broad-spectrum antibiotics: a role for intestinal microbiota

Laranjinha, João, 2019. Nitric Oxide. 27 - 34. 88. 2019. published Nitric Oxide

Red wine extract preserves tight junctions in intestinal epithelial cells under inflammatory conditions: implications for intestinal inflammation

Carla Nunes; Víctor Freitas; Leonor Almeida; João Laranjinha, 2019. Food & Function. 2019. https://doi.org/10.1039/C8FO02469C . 10.1039/C8FO02469C . Food & Function

Simultaneous measurements of ascorbate and glutamate in vivo in the rat brain using carbon fiber nanocomposite sensors and microbiosensor arrays

Laranjinha, João, 2018. Bioelectrochemistry. 121. 2018. published Bioelectrochemistry

Concurrent measurements of neurochemical and electrophysiological activity with microelectrode arrays: New perspectives for constant potential amperometry

Laranjinha, João, 2018. Current Opinion in Electrochemistry. 129 - 140. 12. 2018. published Current Opinion in Electrochemistry

Analysis of respiratory capacity in brain tissue preparations: high-resolution respirometry for intact hippocampal slices

Laranjinha, João, 2018. Analytical Biochemistry. 43 - 50. 551. 2018. published Analytical Biochemistry

Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices

Laranjinha, João, 2018. Methods Mol BIol. 89 - 107. 1782. 2018. published Methods Mol BIol

Age-dependent impairment of neurovascular and neurometabolic coupling in the hippocampus

Laranjinha, João, 2018. Frontiers in Physiology. 9. 2018. published Frontiers in Physiology

Combined in vivo amperometric oximetry and electrophysiology in a single sensor – a tool for epilepsy research

Laranjinha, João, 2017. Analytical Chemistry. 12383 - 12390. 89. 2017. published Analytical Chemistry

Neurometabolic and electrophysiological changes during cortical spreading depolarization: multimodal approach based on a lactate-glucose dual microbiosensor arrays

Laranjinha, João, 2017. Scientific Reports. 1 - 12. 7. 2017. published Scientific Reports

Neurovascular uncoupling in the triple transgenic model of Alzheimer's disease: Impaired cerebral blood flow response to neuronal-derived nitric oxide signaling

Lourenço, C.F.; Ledo, A.; Barbosa, R.M.; Laranjinha, J., 2017. Experimental Neurology. 36 - 43. 291. 2017. http://www.scopus.com/inward/record.url?eid=2-s2.0-85011654048&partnerID=MN8TOARS . 10.1016/j.expneurol.2017.01.013 . Experimental Neurology

Luteolin suppresses the JAK/STAT pathway in a cellular model of intestinal inflammation

Nunes, C.; Almeida, L.; Barbosa, R.M.; Laranjinha, J., 2017. Food and Function. 387 - 396. 1. 8. 2017. http://www.scopus.com/inward/record.url?eid=2-s2.0-85010953233&partnerID=MN8TOARS . 10.1039/c6fo01529h . Food and Function

Neurovascular-neuroenergetic coupling axis in the brain: master regulation by nitric oxide and consequences in aging and neurodegeneration

Lourenço, C.F.; Ledo, A.; Barbosa, R.M.; Laranjinha, J., 2017. Free Radical Biology and Medicine. 668 - 682. 108. 2017. http://www.scopus.com/inward/record.url?eid=2-s2.0-85018262522&partnerID=MN8TOARS . 10.1016/j.freeradbiomed.2017.04.026 . Free Radical Biology and Medicine

Ceramic-based Multi-Site Platinum Microelectrode Arrays: Morphological Characteristics and Electrochemical Performance for Extracellular Oxygen Measurements in Brain Tissue

Laranjinha, João, 2017. Analytical Chemistry. 1674 - 1683. 89. 2017. published Analytical Chemistry

European contribution to the study of ROS: a summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS).

Laranjinha, João, 2017. Redox Biology. 94 - 162. 13. 2017. published Redox Biology

Red wine polyphenol extract protect intestinal epithelial cells from inflammation via complementary modulation of JAK/STAT and Nrf2 pathways

Laranjinha, João, 2016. Toxicology Research. 53 - 65. 5. 2016. published Toxicology Research

Age-dependent changes in the glutamate-nitric oxide pathway in the hippocampus of the triple transgenic model of Alzheimer's disease: implications for neurometabolic regulation

Dias, C.; Lourenço, C.F.; Ferreiro, E.; Barbosa, R.M.; Laranjinha, J.; Ledo, A., 2016. Neurobiology of Aging. 84 - 95. 46. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84978903965&partnerID=MN8TOARS . 10.1016/j.neurobiolaging.2016.06.012 . Neurobiology of Aging

Corrigendum to 'Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain' [Biosens. Bioelectron. 44 (2013) 152-159]. doi: 10.1016/j.bios.2013.01.015.

Santos, R.M.; Rodrigues, M.S.; Laranjinha, J.; Barbosa, R.M., 2016. Biosensors and Bioelectronics. 79. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84956735557&partnerID=MN8TOARS . 10.1016/j.bios.2015.10.086 . Biosensors and Bioelectronics

Tuning constitutive and pathological inflammation in the gut via the interaction of dietary nitrate and polyphenols with host microbiome

Rocha, B.S.; Nunes, C.; Laranjinha, J., 2016. International Journal of Biochemistry and Cell Biology. 393 - 402. 81. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-85001032995&partnerID=MN8TOARS . 10.1016/j.biocel.2016.10.021 . International Journal of Biochemistry and Cell Biology

Microelectrode array biosensor for high-resolution measurements of extracellular glucose in the brain

Lourenço, C.F.; Ledo, A.; Laranjinha, J.; Gerhardt, G.A.; Barbosa, R.M., 2016. Sensors and Actuators, B: Chemical. 298 - 307. 237. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84976594136&partnerID=MN8TOARS . 10.1016/j.snb.2016.06.083 . Sensors and Actuators, B: Chemical

Dietary nitrite induces occludin nitration in the stomach

Rocha, B.S.; Correia, M.G.; Fernandes, R.C.; Gonçalves, J.S.; Laranjinha, J., 2016. Free Radical Research. 1257 - 1264. 11. 50. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84991039527&partnerID=MN8TOARS . 10.1080/10715762.2016.1234049 . Free Radical Research

Role of nitrite, urate and pepsin in the gastroprotective effects of saliva

Rocha, B.S.; Lundberg, J.O.; Radi, R.; Laranjinha, J., 2016. Redox Biology. 407 - 414. 8. 2016. http://www.scopus.com/inward/record.url?eid=2-s2.0-84979517818&partnerID=MN8TOARS . 10.1016/j.redox.2016.04.002 . Redox Biology

Dietary nitrite induces nitrosation of the gastric mucosa: the protective action of the mucus and the modulatory effect of red wine

Cassilda Pereira; Rui M. Barbosa; João Laranjinha, 2015. The Journal of Nutritional Biochemistry. 476 - 483. 5. 26. 2015. https://doi.org/10.1016%2Fj.jnutbio.2014.12.004 . 10.1016/j.jnutbio.2014.12.004 . The Journal of Nutritional Biochemistry

Age-associated changes of nitric oxide concentration dynamics in the central nervous system of Fisher 344 rats.

Laranjinha, João, 2015. Cell Mol Neurobiol. 33 - 44. 35. 2015. published Cell Mol Neurobiol

Ethyl nitrite is produced in the human stomach from dietary nitrate and ethanol, releasing nitric oxide at physiological pH: Potential impact on gastric motility

Rocha, B.S.; Gago, B.; Barbosa, R.M.; Cavaleiro, C.; Laranjinha, J., 2015. Free Radical Biology and Medicine. 160 - 166. 82. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925061584&partnerID=MN8TOARS . 10.1016/j.freeradbiomed.2015.01.021 . Free Radical Biology and Medicine

Red wine polyphenol extract efficiently protects intestinal epithelial cells from inflammation via opposite modulation of JAK/STAT and Nrf2 pathways

Nunes, C.; Teixeira, N.; Serra, D.; Freitas, V.; Almeida, L.; Laranjinha, J., 2015. Toxicology Research. 53 - 65. 1. 5. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84951861397&partnerID=MN8TOARS . 10.1039/c5tx00214a . Toxicology Research

Simultaneous measurement of cholinergic tone and neuronal network dynamics in vivo in the rat brain using a novel choline oxidase based electrochemical biosensor

Santos, R.M.; Laranjinha, J.; Barbosa, R.M.; Sirota, A., 2015. Biosensors and Bioelectronics. 83 - 94. 69. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84923082333&partnerID=MN8TOARS . 10.1016/j.bios.2015.02.003 . Biosensors and Bioelectronics

Coupling of ascorbate and nitric oxide dynamics in vivo in the rat hippocampus upon glutamatergic neuronal stimulation: A novel functional interplay

Ferreira, N.R.; Lourenço, C.F.; Barbosa, R.M.; Laranjinha, J., 2015. Brain Research Bulletin. 13 - 19. 114. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84925442099&partnerID=MN8TOARS . 10.1016/j.brainresbull.2015.03.002 . Brain Research Bulletin

Erratum: Evidence for a pathway that facilitates nitric oxide diffusion in the brain (Neurochem. Int. (2011) 59 (90-96) DOI: 10.1016/j.neuint.2011.05.016)

Santos, R.M.; Lourenço, C.F.; Gerhardt, G.A.; Cadenas, E.; Laranjinha, J.; Barbosa, R.M., 2015. Neurochemistry International. 91. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84951179683&partnerID=MN8TOARS . 10.1016/j.neuint.2015.11.004 . Neurochemistry International

Neurovascular and neurometabolic derailment in aging and alzheimer's disease

Lourenço, C.F.; Ledo, A.; Dias, C.; Barbosa, R.M.; Laranjinha, J., 2015. Frontiers in Aging Neuroscience. MAY. 7. 2015. http://www.scopus.com/inward/record.url?eid=2-s2.0-84931266835&partnerID=MN8TOARS . 10.3389/fnagi.2015.00103 . Frontiers in Aging Neuroscience

The pattern of glutamate-induced nitric oxide dynamics in vivo and its correlation with nNOS expression in rat hippocampus, cerebral cortex and striatum

Lourenço, C.F.; Ferreira, N.R.; Santos, R.M.; Lukacova, N.; Barbosa, R.M.; Laranjinha, J., 2014. Brain Research. 1 - 11. 1554. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84895133025&partnerID=MN8TOARS . 10.1016/j.brainres.2014.01.030 . Brain Research

A shortcut to wide-ranging biological actions of dietary polyphenols: Modulation of the nitrate-nitrite-nitric oxide pathway in the gut

Rocha, B.S.; Nunes, C.; Pereira, C.; Barbosa, R.M.; Laranjinha, J., 2014. Food and Function. 1646 - 1652. 8. 5. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904984090&partnerID=MN8TOARS . 10.1039/c4fo00124a . Food and Function

Neurovascular coupling in hippocampus is mediated via diffusion by neuronal-derived nitric oxide

Lourenço, C.F.; Santos, R.M.; Barbosa, R.M.; Cadenas, E.; Radi, R.; Laranjinha, J., 2014. Free Radical Biology and Medicine. 421 - 429. 73. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84903846214&partnerID=MN8TOARS . 10.1016/j.freeradbiomed.2014.05.021 . Free Radical Biology and Medicine

Nutrition and ageing: From phenomenological observations to molecular mechanisms

Laranjinha, J., 2014. Genes and Nutrition. 4. 9. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84904408748&partnerID=MN8TOARS . 10.1007/s12263-014-0407-5 . Genes and Nutrition

Age-Associated Changes of Nitric Oxide Concentration Dynamics in the Central Nervous System of Fisher 344 Rats

Ledo, A.; Lourenço, C.F.; Caetano, M.; Barbosa, R.M.; Laranjinha, J., 2014. Cellular and Molecular Neurobiology. 33 - 44. 1. 35. 2014. http://www.scopus.com/inward/record.url?eid=2-s2.0-84921938863&partnerID=MN8TOARS . 10.1007/s10571-014-0115-0 . Cellular and Molecular Neurobiology

Real Time In Vivo Measurement of Ascorbate in the Brain Using Carbon Nanotube-Modified Microelectrodes

Ferreira, N.R.; Santos, R.M.; Laranjinha, J.; Barbosa, R.M., 2013. Electroanalysis. 1757 - 1763. 7. 25. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84880348563&partnerID=MN8TOARS . 10.1002/elan.201300053 . Electroanalysis

Human colon adenocarcinoma HT-29 cell: Electrochemistry and nicotine stimulation

Oliveira, S.C.B.; Santarino, I.B.; Enache, T.A.; Nunes, C.; Laranjinha, J.; Barbosa, R.M.; Oliveira-Brett, A.M., 2013. Bioelectrochemistry. 30 - 38. 94. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879324150&partnerID=MN8TOARS . 10.1016/j.bioelechem.2013.05.004 . Bioelectrochemistry

The redox interplay between nitrite and nitric oxide: From the gut to the brain

Pereira, C.; Ferreira, N.R.; Rocha, B.S.; Barbosa, R.M.; Laranjinha, J., 2013. Redox Biology. 276 - 284. 1. 1. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84879005609&partnerID=MN8TOARS . 10.1016/j.redox.2013.04.004 . Redox Biology

Pepsin is nitrated in the rat stomach, acquiring antiulcerogenic activity: A novel interaction between dietary nitrate and gut proteins

Rocha, B.S.; Gago, B.; Barbosa, R.M.; Lundberg, J.O.; Mann, G.E.; Radi, R.; Laranjinha, J., 2013. Free Radical Biology and Medicine. 26 - 34. 58. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84875974388&partnerID=MN8TOARS . 10.1016/j.freeradbiomed.2012.12.017 . Free Radical Biology and Medicine

Biomimetic sensor based on hemin/carbon nanotubes/chitosan modified microelectrode for nitric oxide measurement in the brain

Santos, R.M.; Rodrigues, M.S.; Laranjinha, J.; Barbosa, R.M.; Santos, Ricardo M.; Rodrigues, Marcelo S.; Laranjinha, João; Barbosa, Rui M., 2013. Biosensors and Bioelectronics. 152 - 159. 1. 44. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84873721422&partnerID=MN8TOARS . 10.1016/j.bios.2013.01.015 . Biosensors and Bioelectronics

Intestinal anti-inflammatory activity of red wine extract: Unveiling the mechanisms in colonic epithelial cells

Nunes, C.; Ferreira, E.; Freitas, V.; Almeida, L.; Barbosa, R.M.; Laranjinha, J., 2013. Food and Function. 373 - 383. 3. 4. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874505366&partnerID=MN8TOARS . 10.1039/c2fo30233k . Food and Function

Self-mixing microprobe for monitoring microvascular perfusion in rat brain

Figueiras, E.; Oliveira, R.; Lourenço, C.F.; Campos, R.; Humeau-Heurtier, A.; Barbosa, R.M.; Laranjinha, J.; et al, 2013. Medical and Biological Engineering and Computing. 103 - 112. 1-2. 51. 2013. http://www.scopus.com/inward/record.url?eid=2-s2.0-84874633368&partnerID=MN8TOARS . 10.1007/s11517-012-0973-x . Medical and Biological Engineering and Computing

Intragastric nitration by dietary nitrite: Implications for modulation of protein and lipid signaling

Rocha, B.S.; Gago, B.; Barbosa, R.M.; Lundberg, J.O.; Radi, R.; Laranjinha, J., 2012. Free Radical Biology and Medicine. 693 - 698. 3. 52. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84856266699&partnerID=MN8TOARS . 10.1016/j.freeradbiomed.2011.11.011 . Free Radical Biology and Medicine

Critical role of hydrogen peroxide signaling in the sequential activation of p38 MAPK and eNOS in laminar shear stress

Bretón-Romero, R.; González De Orduña, C.; Romero, N.; Sánchez-Gómez, F.J.; De Álvaro, C.; Porras, A.; Rodríguez-Pascual, F.; et al, 2012. Free Radical Biology and Medicine. 1093 - 1100. 6. 52. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84857233171&partnerID=MN8TOARS . 10.1016/j.freeradbiomed.2011.12.026 . Free Radical Biology and Medicine

Nitric oxide signaling in the brain: Translation of dynamics into respiration control and neurovascular coupling

Laranjinha, J.; Santos, R.M.; Lourenço, C.F.; Ledo, A.; Barbosa, R.M., 2012. Annals of the New York Academy of Sciences. 10 - 18. 1. 1259. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863518007&partnerID=MN8TOARS . 10.1111/j.1749-6632.2012.06582.x . Annals of the New York Academy of Sciences

Nitric oxide inactivation mechanisms in the brain: Role in bioenergetics and neurodegeneration

Santos, R.M.; Lourenço, C.F.; Ledo, A.; Barbosa, R.M.; Laranjinha, J., 2012. International Journal of Cell Biology. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-84863706810&partnerID=MN8TOARS . 10.1155/2012/391914 . International Journal of Cell Biology

Modulation of cellular respiration by endogenously produced nitric oxide in rat hippocampal slices

Ledo, A.; Barbosa, R.M.; Laranjinha, J., 2012. Methods in Molecular Biology. 73 - 88. 810. 2012. http://www.scopus.com/inward/record.url?eid=2-s2.0-82355193799&partnerID=MN8TOARS . 10.1007/978-1-61779-382-0_5 . Methods in Molecular Biology

Preparation, standardization and measurement of nitric oxide solution

Laranjinha, João, 2011. Global Journal Analytical Chemistry . 272 - 284. 2. 2011. published Global Journal Analytical Chemistry

Brain nitric oxide inactivation is governed by the vasculature

Santos, R.M.; Lourenço, C.F.; Pomerleau, F.; Huettl, P.; Gerhardt, G.A.; Laranjinha, J.; Barbosa, R.M., 2011. Antioxidants and Redox Signaling. 1011 - 1021. 6. 14. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79951864475&partnerID=MN8TOARS . 10.1089/ars.2010.3297 . Antioxidants and Redox Signaling

In vivo modulation of nitric oxide concentration dynamics upon glutamatergic neuronal activation in the hippocampus

LourençO, C.F.; Santos, R.; Barbosa, R.M.; Gerhardt, G.; Cadenas, E.; Laranjinha, J., 2011. Hippocampus. 622 - 630. 6. 21. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79956360575&partnerID=MN8TOARS . 10.1002/hipo.20774 . Hippocampus

Dietary nitrite in nitric oxide biology: A redox interplay with implications for pathophysiology and therapeutics

Rocha, B.S.; Gago, B.; Pereira, C.; Barbosa, R.M.; Bartesaghi, S.; Lundberg, J.O.; Radi, R.; Laranjinha, J., 2011. Current Drug Targets. 1351 - 1363. 9. 12. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79959998237&partnerID=MN8TOARS . 10.2174/138945011796150334 . Current Drug Targets

Evidence for a pathway that facilitates nitric oxide diffusion in the brain

Santos, R.M.; Loureno, C.F.; Gerhardt, G.A.; Cadenas, E.; Laranjinha, J.; Barbosa, R.M., 2011. Neurochemistry International. 90 - 96. 1. 59. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79960196903&partnerID=MN8TOARS . 10.1016/j.neuint.2011.05.016 . Neurochemistry International

Nitric oxide and DOPAC-induced cell death: From GSH depletion to mitochondrial energy crisis

Nunes, C.; Barbosa, R.M.; Almeida, L.; Laranjinha, J., 2011. Molecular and Cellular Neuroscience. 94 - 103. 1. 48. 2011. http://www.scopus.com/inward/record.url?eid=2-s2.0-79960921750&partnerID=MN8TOARS . 10.1016/j.mcn.2011.06.009 . Molecular and Cellular Neuroscience

Dynamic and interacting profiles of .NO and O2 in rat hippocampal slices

Ledo, A.; Barbosa, R.; Cadenas, E.; Laranjinha, J., 2010. Free Radical Biology and Medicine. 1044 - 1050. 8. 48. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-77950516684&partnerID=MN8TOARS . 10.1016/j.freeradbiomed.2010.01.024 . Free Radical Biology and Medicine

Diffusion of nitric oxide through the gastric wall upon reduction of nitrite by red wine: Physiological impact

Rocha, B.S.; Gago, B.; Barbosa, R.M.; Laranjinha, J., 2010. Nitric Oxide - Biology and Chemistry. 235 - 241. 3. 22. 2010. http://www.scopus.com/inward/record.url?eid=2-s2.0-76949084897&partnerID=MN8TOARS . 10.1016/j.niox.2010.01.003 . Nitric Oxide - Biology and Chemistry

Stimulation of NMDA and AMPA glutamate receptors elicits distinct concentration dynamics of nitric oxide in rat hippocampal slices

Frade, J.G.; Barbosa, R.M.; Laranjinha, J., 2009. Hippocampus. 603 - 611. 7. 19. 2009. http://www.scopus.com/inward/record.url?eid=2-s2.0-67649667029&partnerID=MN8TOARS . 10.1002/hipo.20536 . Hippocampus

Dietary polyphenols generate nitric oxide from nitrite in the stomach and induce smooth muscle relaxation

Rocha, B.S.; Gago, B.; Barbosa, R.M.; Laranjinha, J., 2009. Toxicology. 41 - 48. 1-2. 265. 2009. http://www.scopus.com/inward/record.url?eid=2-s2.0-70349876563&partnerID=MN8TOARS . 10.1016/j.tox.2009.09.008 . Toxicology

Diphenyl diselenide, a simple glutathione peroxidase mimetic, inhibits human LDL oxidation in vitro

Bem, Andreza Fabro de; Farina, Marcelo; Portella, Rafael de Lima; Nogueira, Cristina Wayne; Dinis, Teresa C. P.; Laranjinha, João A. N.; Almeida, Leonor M.; et al, 2008. Atherosclerosis. 92 - 100. 1. 201. 2008. http://hdl.handle.net/10316/5839 . 10.1016/j.atherosclerosis.2008.02.030 . Atherosclerosis

In Vivo Real-Time Measurement of Nitric Oxide in Anesthetized Rat Brain

Barbosa, R.M.; Lourenço, C.F.; Santos, R.M.; Pomerleau, F.; Huettl, P.; Gerhardt, G.A.; Laranjinha, J., 2008. Methods in Enzymology. 351 - 367. 441. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-52049109388&partnerID=MN8TOARS . 10.1016/S0076-6879(08)01220-2 . published Methods in Enzymology

A comparative study of carbon fiber-based microelectrodes for the measurement of nitric oxide in brain tissue

Santos, R.M.; Lourenço, C.F.; Piedade, A.P.; Andrews, R.; Pomerleau, F.; Huettl, P.; Gerhardt, G.A.; Laranjinha, J.; Barbosa, R.M., 2008. Biosensors and Bioelectronics. 704 - 709. 4. 24. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-53049092283&partnerID=MN8TOARS . 10.1016/j.bios.2008.06.034 . Biosensors and Bioelectronics

LDL isolated from plasma-loaded red wine procyanidins resist lipid oxidation and tocopherol depletion

Lourenço, C.F.; Gago, B.; Barbosa, R.M.; De Freitas, V.; Laranjinha, J., 2008. Journal of Agricultural and Food Chemistry. 3798 - 3804. 10. 56. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-45549089760&partnerID=MN8TOARS . 10.1021/jf0733259 . Journal of Agricultural and Food Chemistry

3,4-Dihydroxyphenylacetic acid (DOPAC) modulates the toxicity induced by nitric oxide in PC-12 cells via mitochondrial dysfunctioning

Nunes, C.; Almeida, L.; Laranjinha, J.; Nunes, Carla; Almeida, Leonor; Laranjinha, João, 2008. NeuroToxicology. 998 - 1007. 6. 29. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-56249095456&partnerID=MN8TOARS . 10.1016/j.neuro.2008.07.003 . NeuroToxicology

Glutamate induces release of glutathione from cultured rat astrocytes - A possible neuroprotective mechanism?

Frade, J.; Pope, S.; Schmidt, M.; Dringen, R.; Barbosa, R.; Pocock, J.; Laranjinha, J.; Heales, S., 2008. Journal of Neurochemistry. 1144 - 1152. 4. 105. 2008. http://www.scopus.com/inward/record.url?eid=2-s2.0-42549146494&partnerID=MN8TOARS . 10.1111/j.1471-4159.2008.05216.x . Journal of Neurochemistry

The potent vasodilator ethyl nitrite is formed upon reaction of nitrite and ethanol under gastric conditions

Gago, Bruno; Nyström, Thomas; Cavaleiro, Carlos; Rocha, Bárbara S.; Barbosa, Rui M.; Laranjinha, João; Lundberg, Jon O.; et al, 2008. Free Radical Biology and Medicine. 404 - 412. 4. 45. 2008. http://hdl.handle.net/10316/5829 . 10.1016/j.freeradbiomed.2008.04.027 . Free Radical Biology and Medicine

NO-mediated apoptosis in yeast

Almeida, B.; Buttner, S.; Ohlmeier, S.; Silva, A.; Mesquita, A.; Sampaio-Marques, B.; Osório, N.S.; et al, 2007. Journal of Cell Science. 3279 - 3288. 18. 120. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-35548961293&partnerID=MN8TOARS . 10.1242/jcs.010926 . Journal of Cell Science

Coordination of physiologic and toxic pathways in hippocampus by nitric oxide and mitochondria

Laranjinha, J.; Ledo, A., 2007. Frontiers in Bioscience. 1094 - 1106. 3. 12. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34249799636&partnerID=MN8TOARS . 10.2741/2129 . Frontiers in Bioscience

Nitric oxide signaling is disrupted in the yeast model for Batten disease

Osório, N.S.; Carvalho, A.; Almeida, A.J.; Padilla-Lopez, S.; Leão, C.; Laranjinha, J.; Ludovico, P.; Pearce, D.A.; Rodrigues, F., 2007. Molecular Biology of the Cell. 2755 - 2767. 7. 18. 2007. http://www.scopus.com/inward/record.url?eid=2-s2.0-34347398122&partnerID=MN8TOARS . 10.1091/mbc.E06-11-1053 . Molecular Biology of the Cell

Red wine-dependent reduction of nitrite to nitric oxide in the stomach

Gago, Bruno; Lundberg, Jon O.; Barbosa, Rui M.; Laranjinha, João; Gago, B.; Lundberg, J.O.; Barbosa, R.M.; Laranjinha, J., 2007. Free Radical Biology and Medicine. 1233 - 1242. 9. 43. 2007. http://hdl.handle.net/10316/5851 . 10.1016/j.freeradbiomed.2007.06.007 . Free Radical Biology and Medicine

Regulation of nitric oxide dynamics in hippocampus by NMDA and AMPA glutamate receptors

Ledo, A.; Frade, J.G.; Barbosa, R.M.; Laranjinha, J., 2006. Current Topics in Pharmacology. 55 - 70. 2. 10. 2006. http://www.scopus.com/inward/record.url?eid=2-s2.0-42149182950&partnerID=MN8TOARS . Current Topics in Pharmacology

Dietary flavonoids with a catechol structure increase a-tocopherol in rats and protect the vitamin from oxidation in vitro1

Frank, J.; Budek, A.; Lundh, T.; Parker, R.S.; Swanson, J.E.; Lourenço, C.F.; Gago, B.; et al, 2006. Journal of Lipid Research. 2718 - 2725. 12. 47. 2006. http://www.scopus.com/inward/record.url?eid=2-s2.0-33845570860&partnerID=MN8TOARS . 10.1194/jlr.M600291-JLR200 . Journal of Lipid Research

Redox interactions of nitric oxide with dopamine and its derivatives

Antunes, Fernando; Nunes, Carla; Laranjinha, João; Cadenas, Enrique; Antunes, F.; Nunes, C.; Laranjinha, J.; Cadenas, E., 2005. Toxicology. 207 - 212. 2. 208. 2005. http://hdl.handle.net/10316/5757 . 10.1016/j.tox.2004.11.033 . Toxicology

Concentration dynamics of nitric oxide in rat hippocampal subregions evoked by stimulation of the NMDA glutamate receptor

Ledo, Ana; Barbosa, Rui M.; Gerhardt, Greg A.; Cadenas, Enrique; Laranjinha, João; Ledo, A.; Barbosa, R.M.; et al, 2005. Proceedings of the National Academy of Sciences of the United States of America. 17483 - 17488. 48. 102. 2005. http://hdl.handle.net/10316/12779 . 10.1073/pnas.0503624102 . Proceedings of the National Academy of Sciences of the United States of America

Scavenging of nitric oxide by an antagonist of adenosine receptors

Sousa, T.; Fernandes, E.; Nunes, C.; Laranjinha, J.; Carvalho, F.; Pinho, D.; Morato, M.; Albino-Teixeira, A., 2005. Journal of Pharmacy and Pharmacology. 399 - 404. 3. 57. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-14644418399&partnerID=MN8TOARS . 10.1211/0022357055614 . Journal of Pharmacy and Pharmacology

Concentration Dynamics of Nitric Oxide in Rat Hippocampal Slices

Ledo, Ana; Barbosa, Rui M.; Cadenas, Enrique; Gerhardt, Greg; Laranjinha, João, 2005. 2005. http://hdl.handle.net/10316/9661 .

Synergistic inhibition of respiration in brain mitochondria by nitric oxide and dihydroxyphenylacetic acid (DOPAC): Implications for Parkinson's disease

Nunes, C.; Almeida, L.; Laranjinha, J., 2005. Neurochemistry International. 173 - 182. 3. 47. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-20544459248&partnerID=MN8TOARS . 10.1016/j.neuint.2005.03.005 . Neurochemistry International

Electrochemical measurement of endogenously produced nitric oxide in brain slices using Nafion/o-phenylenediamine modified carbon fiber microelectrodes

Ferreira, N.R.; Ledo, A.; Frade, J.G.; Gerhardt, G.A.; Laranjinha, J.; Barbosa, R.M.; Ferreira, Nuno R.; et al, 2005. Analytica Chimica Acta. 1 - 7. 1-2. 535. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-15744400539&partnerID=MN8TOARS . 10.1016/j.aca.2004.12.017 . Analytica Chimica Acta

Mechanisms of neuroprotection by polyphenols

Frade, J.G.; Ferreira, N.R.; Barbosa, R.M.; Laranjinha, J., 2005. Current Medicinal Chemistry - Central Nervous System Agents. 307 - 318. 4. 5. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-28244468104&partnerID=MN8TOARS . 10.2174/156801505774913062 . Current Medicinal Chemistry - Central Nervous System Agents

Kinetics and thermodynamics of lipid amphiphile exchange between lipoproteins and albumin in serum

Estronca, L.M.B.B.; Moreno, M.J.; Laranjinha, J.A.N.; Almeida, L.M.; Vaz, W.L.C., 2005. Biophysical Journal. 557 - 565. 1. 88. 2005. http://www.scopus.com/inward/record.url?eid=2-s2.0-11244253621&partnerID=MN8TOARS . 10.1529/biophysj.104.047050 . Biophysical Journal

Nitric oxide in brain: Diffusion, targets and concentration dynamics in hippocampal subregions

Ledo, A.; Frade, J.; Barbosa, R.M.; Laranjinha, J.; Ledo, Ana; Frade, João; Barbosa, Rui M.; Laranjinha, João, 2004. Molecular Aspects of Medicine. 75 - 89. 1-2. 25. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-1642422744&partnerID=MN8TOARS . 10.1016/j.mam.2004.02.010 . Molecular Aspects of Medicine

Simultaneous production of superoxide radical and singlet oxygen by sulphonated chloroaluminum phthalocyanine incorporated in human low-density lipoproteins: Implications for photodynamic therapy

Martins, J.; Almeida, L.; Laranjinha, J., 2004. Photochemistry and Photobiology. 267 - 273. 2. 80. 2004. http://www.scopus.com/inward/record.url?eid=2-s2.0-9144273256&partnerID=MN8TOARS . 10.1562/2004-03-26-RA-124.1 . Photochemistry and Photobiology

Antioxidant protection of low density lipoprotein by procyanidins: Structure/activity relationships

Da Silva Porto, P.A.L.; Laranjinha, J.A.N.; De Freitas, V.A.P.; Porto, Patrícia Andréia Leite da Silva; Laranjinha, João António Nave; Freitas, Victor Armando Pereira de, 2003. Biochemical Pharmacology. 947 - 954. 6. 66. 2003. http://www.scopus.com/inward/record.url?eid=2-s2.0-0042261671&partnerID=MN8TOARS . 10.1016/S0006-2952(03)00458-1 . Biochemical Pharmacology

Nitric oxide monitoring in hippocampal brain slices using electrochemical methods

Ledo, A.; Barbosa, R.M.; Frade, J.; Laranjinha, J., 2002. Methods in Enzymology. 111 - 125. 359. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-18744413028&partnerID=MN8TOARS . 10.1016/S0076-6879(02)59176-X . Methods in Enzymology

Oxidation of DOPAC by nitric oxide: Effect of superoxide dismutase

Laranjinha, J.; Cadenas, E.; Laranjinha, João; Cadenas, Enrique, 2002. Journal of Neurochemistry. 892 - 900. 4. 81. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036316430&partnerID=MN8TOARS . 10.1046/j.1471-4159.2002.00900.x . Journal of Neurochemistry

Photoactivation of phthalocyanine-loaded low density lipoproteins induces a local oxidative stress that propagates to human erythrocytes: Protection by caffeic acid

Martins, J.; Madeira, V.; Almeida, L.; Laranjinha, J., 2002. Free Radical Research. 319 - 328. 3. 36. 2002. http://www.scopus.com/inward/record.url?eid=2-s2.0-0036521948&partnerID=MN8TOARS . 10.1080/10715760290019345 . Free Radical Research

Redox cycles of caffeic acid with a-tocopherol and ascorbate

Laranjinha, J., 2001. Methods in Enzymology. 282 - 295. 335. 2001. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034981221&partnerID=MN8TOARS . 10.1016/S0076-6879(01)35251-5 . Methods in Enzymology

Inhibition of human LDL lipid peroxidation by phenol-rich beverages and their impact on plasma total antioxidant capacity in humans

Serafini, M.; Laranjinha, J.A.N; Almeida, L.M.; Maiani, G.; Serafini, Mauro; Laranjinha, João A. N.; Almeida, Leonor M., 2000. Journal of Nutritional Biochemistry. 585 - 590. 11-12. 11. 2000. http://www.scopus.com/inward/record.url?eid=2-s2.0-0034541445&partnerID=MN8TOARS . 10.1016/S0955-2863(00)00124-8 . Journal of Nutritional Biochemistry

Redox cycles of caffeic acid, a-tocopherol, and ascorbate: Implications for protection of low-density lipoproteins against oxidation

Laranjinha, J.; Cadenas, E., 1999. IUBMB Life. 57 - 65. 1. 48. 1999. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032744791&partnerID=MN8TOARS . 10.1080/152165499307422 . IUBMB Life

Redox Cycles of Caffeic Acid, alpha-Tocopherol, and Ascorbate: Implications for Protection of Low-Density Lipoproteins Against Oxidation

Laranjinha, João; Cadenas, Enrique, 1999. 1999. http://hdl.handle.net/10316/8366 .

Effect of dietary phenolic compounds on apoptosis of human cultured endothelial cells induced by oxidized LDL

Vieira, O.; Escargueil-Blanc, I.; Meilhac, O.; Basile, J.-P.; Laranjinha, J.; Almeida, L.; Salvayre, R.; Nègre-Salvayre, A., 1998. British Journal of Pharmacology. 565 - 573. 3. 123. 1998. http://www.scopus.com/inward/record.url?eid=2-s2.0-0031965148&partnerID=MN8TOARS . 10.1038/sj.bjp.0701624 . British Journal of Pharmacology

Cholesteryl ester hydroperoxide formation in myoglobin-catalyzed low density lipoprotein oxidation. Concerted antioxidant activity of caffeic and p-coumaric acids with ascorbate

Vieira, O.; Laranjinha, J.; Madeira, V.; Almeida, L., 1998. Biochemical Pharmacology. 333 - 340. 3. 55. 1998. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032007530&partnerID=MN8TOARS . 10.1016/S0006-2952(97)00470-X . Biochemical Pharmacology

Sulfonated Chloroaluminum Phthalocyanine Incorporates into Human Plasma Lipoproteins: Photooxidation of Low-Density Lipoproteins

Santos, A.E.; Laranjinha, J.A.N.; Almeida, L.M., 1998. Photochemistry and Photobiology. 378 - 385. 4. 67. 1998. http://www.scopus.com/inward/record.url?eid=2-s2.0-0032040364&partnerID=MN8TOARS . Photochemistry and Photobiology

Inhibition of metmyoglobin/H2O2-dependent low density lipoprotein lipid peroxidation by naturally occurring phenolic acids

Laranjinha, J.; Vieira, O.; Almeida, L.; Madeira, V., 1996. Biochemical Pharmacology. 395 - 402. 4. 51. 1996. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029664717&partnerID=MN8TOARS . 10.1016/0006-2952(95)02171-X . Biochemical Pharmacology

Rapid isolation of low density lipoproteins in a concentrated fraction free from water-soluble plasma antioxidants

Vieira, Otília V.; Laranjinha, João A.; Madeira, Vítor M.; Almeida, Leonor M.; Vieira, O.V.; Laranjinha, J.A.N.; Madeira, V.M.C.; Almeida, L.M., 1996. Journal of Lipid Research. 2715 - 2721. 12. 37. 1996. http://hdl.handle.net/10316/12736 . Journal of Lipid Research

Two related phenolic antioxidants with opposite effects on vitamin E content in low density lipoproteins oxidized by ferrylmyoglobin: Consumption vs regeneration

Laranjinha, J.; Vieira, O.; Madeira, V.; Almeida, L., 1995. Archives of Biochemistry and Biophysics. 373 - 381. 2. 323. 1995. http://www.scopus.com/inward/record.url?eid=2-s2.0-0028840619&partnerID=MN8TOARS . 10.1006/abbi.1995.0057 . Archives of Biochemistry and Biophysics

Reduction of ferrylmyoglobin by dietary phenolic acid derivatives of cinnamic acid

Laranjinha, J.; Almeida, L.; Madeira, V., 1995. Free Radical Biology and Medicine. 329 - 337. 3. 19. 1995. http://www.scopus.com/inward/record.url?eid=2-s2.0-0029160111&partnerID=MN8TOARS . 10.1016/0891-5849(95)00039-Z . Free Radical Biology and Medicine

Reactivity of dietary phenolic acids with peroxyl radicals: Antioxidant activity upon low density lipoprotein peroxidation

Laranjinha, J.A.N.; Almeida, L.M.; Madeira, V.M.C., 1994. Biochemical Pharmacology. 487 - 494. 3. 48. 1994. http://www.scopus.com/inward/record.url?eid=2-s2.0-0027982195&partnerID=MN8TOARS . 10.1016/0006-2952(94)90278-X . Biochemical Pharmacology

Lipid peroxidation and its inhibition in low density lipoproteins: Quenching of cis-parinaric acid fluorescence

Laranjinha, J.A.N.; Almeida, L.M.; Madeira, V.M.C., 1992. Archives of Biochemistry and Biophysics. 147 - 154. 1. 297. 1992. http://www.scopus.com/inward/record.url?eid=2-s2.0-0026782042&partnerID=MN8TOARS . 10.1016/0003-9861(92)90653-E . Archives of Biochemistry and Biophysics

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