Neuroscience and Disease

Axonal biology in neurodevelopment and neurodegeneration

Axons, Alzheimer's disease, Autophagy, Rare diseases, Neurodevelopment.

Research lines

To characterize the role of heterogeneous nuclear ribonucleoproteins (hnRNPs) in neurodevelopmental disorders

To study the role of intra-axonal autophagy in the pathogenesis of Alzheimer’s disease


The laboratory research focuses on intra-axonal and dendritic signalling pathways, in hippocampal and cortical neurons, under physiological and pathological conditions. In particular, the research group aims to study the involvement of intra-axonal autophagy dysfunction in Alzheimer’s disease onset and understand the role of RNA-binding proteins in rare neurodevelopmental disorders and epilepsy.
The techniques applied in this laboratory include primary cell cultures, in vivo transfection of nucleic acids (in utero electroporation), in vitro and in vivo pharmacology, biochemical assays, immunocytochemistry, and confocal (live-)imaging.
These strategies allow a solid study of neuronal development and the mechanisms underlying brain neurodegenerative disorders, aiming at the development of new therapeutical approaches.


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Information about journal articles, updated at 24-09-2023, from platform CIÊNCIAVITAE.

Brain-derived neurotrophic factor -induced regulation of RNA metabolism in neuronal development and synaptic plasticity

Rui O. Costa; Luís F. Martins; Emanuel Tahiri; Carlos B. Duarte, 2022. WIREs RNA. 2022. . 10.1002/wrna.1713 . WIREs RNA

Isolation and Culture of Chick Ciliary Ganglion Neurons

Costa, Filipa J.; Dias, Marta S.; Costa, Rui O.; Pedro, Joana R.; Almeida, Ramiro D., 2020. Journal of Visualized Experiments. 162. 2020. . 10.3791/61431 . Journal of Visualized Experiments

Synaptogenesis Stimulates a Proteasome-Mediated Ribosome Reduction in Axons

Costa, Rui O.; Martins, Helena; Martins, Luís F.; Cwetsch, Andrzej W.; Mele, Miranda; Pedro, Joana R.; Tomé, Diogo; et al, 2019. Cell Reports. 864 - 876.e6. 4. 28. 2019. . 10.1016/j.celrep.2019.06.080 . Cell Reports

Alterations in GABAA-Receptor Trafficking and Synaptic Dysfunction in Brain Disorders

Mele, Miranda; Costa, Rui O.; Duarte, Carlos B., 2019. Frontiers in Cellular Neuroscience. 13. 2019. . 10.3389/fncel.2019.00077 . Frontiers in Cellular Neuroscience

Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF

Martins, L.F.; Costa, R.O.; Pedro, J.R.; Aguiar, P.; Serra, S.C.; Teixeira, F.G.; Sousa, N.; Salgado, A.J.; Almeida, R.D., 2017. Scientific Reports. 1. 7. 2017. . 10.1038/s41598-017-03592-1 . Scientific Reports

The RNA-Binding Protein hnRNP K Mediates the Effect of BDNF on Dendritic mRNA Metabolism and Regulates Synaptic NMDA Receptors in Hippocampal Neurons

Graciano Leal; Diogo Comprido; Pasqualino de Luca; Eduardo Morais; Luís Rodrigues; Miranda Mele; Ana R. Santos; et al, 2017. eneuro. ENEURO.0268 - 17.2017. 6. 4. 2017. . 10.1523/ENEURO.0268-17.2017 . eneuro

Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons

Salazar, Ivan; Mele, Miranda; Caldeira, Margarida; Costa, Rui; Correia, Bárbara; Frisari, Simone; Duarte, Carlos; Costa RO, 2017. BIO-PROTOCOL. 18. 7. 2017. . 10.21769/bioprotoc.2551 . BIO-PROTOCOL

PROneurotrophins and CONSequences

Costa, R.O.; Perestrelo, T.; Almeida, R.D., 2017. Molecular Neurobiology. 1 - 18. 2017. . 10.1007/s12035-017-0505-7 . Molecular Neurobiology

Visualizing K48 ubiquitination during presynaptic formation by ubiquitination-induced fluorescence complementation (UiFC)

Pinto, M.J.; Pedro, J.R.; Costa, R.O.; Almeida, R.D., 2016. Frontiers in Molecular Neuroscience. JUNE. 9. 2016. . 10.3389/fnmol.2016.00043 . Frontiers in Molecular Neuroscience

Oxidative stress involving changes in Nrf2 and ER stress in early stages of Alzheimer's disease

Costa, R.O.; Mota, S.I.; Ferreira, I.L.; Santana, I.; Caldeira, G.L.; Padovano, C.; Fonseca, A.C.; et al, 2015. Biochimica et Biophysica Acta - Molecular Basis of Disease. 1428 - 1441. 7. 1852. 2015. . 10.1016/j.bbadis.2015.03.015 . Biochimica et Biophysica Acta - Molecular Basis of Disease

Inhibition of mitochondrial cytochrome c oxidase potentiates Aß-induced ER stress and cell death in cortical neurons

Costa, R.O.; Ferreiro, E.; Oliveira, C.R.; Pereira, C.M.F., 2013. Molecular and Cellular Neuroscience. 1 - 8. 52. 2013. . 10.1016/j.mcn.2012.09.005 . Molecular and Cellular Neuroscience

Leptin and ghrelin prevent hippocampal dysfunction induced by Aß oligomers

Martins, I.; Gomes, S.; Costa, R.O.; Otvos, L.; Oliveira, C.R.; Resende, R.; Pereira, C.M.F., 2013. Neuroscience. 41 - 51. 241. 2013. . 10.1016/j.neuroscience.2013.02.062 . Neuroscience

Endoplasmic reticulum stress occurs downstream of GluN2B subunit of N-methyl-d-aspartate receptor in mature hippocampal cultures treated with amyloid-ß oligomers

Costa, R.O.; Lacor, P.N.; Ferreira, I.L.; Resende, R.; Auberson, Y.P.; Klein, W.L.; Oliveira, C.R.; Rego, A.C.; Pereira, C.M.F., 2012. Aging Cell. 823 - 833. 5. 11. 2012. . 10.1111/j.1474-9726.2012.00848.x . Aging Cell

Amyloid ß-induced ER stress is enhanced under mitochondrial dysfunction conditions

Costa, R.O.; Ferreiro, E.; Martins, I.; Santana, I.; Cardoso, S.M.; Oliveira, C.R.; Pereira, C.M.F., 2012. Neurobiology of Aging. 4. 33. 2012. . 10.1016/j.neurobiolaging.2011.04.011 . Neurobiology of Aging

Mitochondrial- and endoplasmic reticulum-associated oxidative stress in alzheimers disease: From pathogenesis to biomarkers

Ferreiro, E.; Baldeiras, I.; Ferreira, I.L.; Costa, R.O.; Rego, A.C.; Pereira, C.F.; Oliveira, C.R., 2012. International Journal of Cell Biology. 2012. . 10.1155/2012/735206 . International Journal of Cell Biology

ER stress-mediated apoptotic pathway induced by aß peptide requires the presence of functional mitochondria

Costa, R.O.; Ferreiro, E.; Cardoso, S.M.; Oliveira, C.R.; Pereira, C.M.F., 2010. Journal of Alzheimer's Disease. 625 - 636. 2. 20. 2010. . 10.3233/JAD-2010-091369 . Journal of Alzheimer's Disease

Involvement of mitochondria in endoplasmic reticulum stress-induced apoptotic cell death pathway triggered by the prion peptide PrP 106-126

Ferreiro, E.; Costa, R.; Marques, S.; Cardoso, S.M.; Oliveira, C.R.; Pereira, C.M.F., 2008. Journal of Neurochemistry. 766 - 776. 3. 104. 2008. . 10.1111/j.1471-4159.2007.05048.x . Journal of Neurochemistry

An endoplasmic-reticulum-specific apoptotic pathway is involved in prion and amyloid-beta peptides neurotoxicity

Ferreiro, E.; Resende, R.; Costa, R.; Oliveira, C.R.; Pereira, C.M.F., 2006. Neurobiology of Disease. 669 - 678. 3. 23. 2006. . 10.1016/j.nbd.2006.05.011 . Neurobiology of Disease



Scientific Incentive Grant

Portuguese Epilepsy foundation (LPCE)

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