The Flow Cytometry Unit provide a range of services, including sample analysis and cell sorting, as well as equipment, software, and technical expertise/advice for a variety of flow cytometric protocols.
Its function is to provide high quality, cost effective state-of-the-art flow cytometry and multiparameter cell sorting to all investigators at CNC and serves to enhance the quality and scope of scientific research performed at CNC.
The unit has a state of the art cell sorter FACSAria III, where as much as four different cell populations can be sorted simultaneously, and cells can either be sorted onto slides, into tubes (1-15 ml) or plates (6-384 wells). In addition, we have a Partec CyFlow space that sort one population at a time, and a FACSCalibur and an Accuri C6 analyser, which can perform multi-parameter flow cytometric analysis.
Training is available to enable users to run analytical cytometers themselves. The cell analysers are available 7 days a week-24 hours a day for trained users. The more complex cell sorting service requires a dedicated operator and is open for use during normal Monday to Friday working hours.
The facility is divided in two places: Polo I at Coimbra and UC-Biotech at Cantanhede. Inquiries concerning access to the facility should be directed to any of the facility staff.
The flow cytometry unit is available to all CNC researchers, as well as to other interested academic or commercial users, after prior arrangement.
Guidelines for the use of the facility are provided on these web pages. Please ensure you have read the relevant guidelines to maximize the success and validity of your experiment.
Keywords:
Flow cytometry, cell sorting, cell analysis.
Services / Technologies:
Cell sorting;
Data acquisition, analysis and interpretation;
User training;
Advice on sample preparation;
User assistance and machine troubleshooting;
Help with design of experiments;
Sourcing of reagents and techniques and finding alternative approaches;
Help with data preparation for presentation and publication;
We are also open to collaboration.
List of Available Equipment:
Becton Dickinson FACSCalibur cell analyser (Polo I);
Partec CyFlow® Space cell sorter (Polo I);
Becton Dickinson Accuri™ C6 cell analyser (UC-Biotech) with auto-sampler;
Becton Dickinson FACSAria III cell sorter (UC-Biotech).
Contact:
flow-poloI@cnc.uc.pt (Polo I) or flow@uc-biotech.pt (UC-Biotech)
