Biomaterials and Stem Cell-Based Therapeutics


Cardiovascular Diseases

Stem Cells

iPSCs Differentiation


Research lines

Disease modeling and drug screening programs based on stem cell-based models

Nanomedicine program to modulate the activity of stem cells and their progenies


The research group of biomaterials and stem cell-based therapeutics was created in January 2008 at CNC. The research group aims at generating fundamental and translational knowledge in the intersection of biomaterials with stem cells. The research group has two main avenues of research:

  1. Disease modeling and drug screening program: in vitro cell/tissue models from human stem cells. Stem cells, in particular induced pluripotent stem cells (iPSCs), may be an excellent source of cells for disease modeling and drug discovery programs related to cardiovascular diseases. The potential of iPSCs to generate disease models led to the creation of several biobanks in USA (Coriell Institute for Medical Research, NIH Center for Regenerative Medicine, ATCC and University owned biobanks), Europe (Cellartis; and an European initiative of Stem cell biobank) and Japan (RIKEN bioresource center) for storage and distribution of iPSC lines originated from patients and healthy controls. In the last 6 years my research group has developed several tissue models from stem cells that may be an important platform for drug discovery programs related to cardiovascular diseases. A particular interest of the group is to develop biomaterials and bioengineering platforms for the efficient maturation/specification of stem cells and their progenies. The research group uses many tools to accomplish this goal, including the design of new biomaterials with relevant biological information, molecular and cell biology, microfluidic systems, high content analysis, and animal experimentation.
  2. Nanomedicine platforms to modulate the activity of stem cells and their progenies. The development of a wide spectrum of nanotechnologies (referred as Nanomedicine by National Institutes of Health for applications in the biomedical area) during the last years are very promising for the study of stem cell biology and to control exogenous and endogenous stem cells for regenerative medicine. Our group is particularly interested to use these tools to induce in vivo stem cell differentiation and to mobilize stem cells from their niches to treat cardiovascular diseases. For this purpose, we are developing nanomaterials that release efficiently biomolecules (small molecules, proteins or non-coding RNAs) to manipulate stem cells or their progenies.


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Information about journal articles, updated at 25-07-2021, from platform CIÊNCIAVITAE.

Development of an optimized and scalable method for isolation of umbilical cord blood-derived small extracellular vesicles for future clinical use

Renato M. S. Cardoso; Silvia C. Rodrigues; Claudia F. Gomes; Filipe V. Duarte; Maryse Romao; Ermelindo C. Leal; Patricia C. Freire; Ricardo Neves; Joana Simões-Correia, 2021. STEM CELLS Translational Medicine. 2021. . 10.1002/sctm.20-0376 . STEM CELLS Translational Medicine

Correction to: In vitro mechanistic studies on a-amanitin and its putative antidotes

Daniela Ferreira Rodrigues; Ricardo Pires das Neves; Alexandra T. P. Carvalho; Maria Lourdes Bastos; Vera M. Costa; Félix Carvalho, 2020. Archives of Toxicology. 2020. . 10.1007/s00204-020-02735-0 . Archives of Toxicology

In vitro mechanistic studies on a-amanitin and its putative antidotes

Daniela Ferreira Rodrigues; Ricardo Pires das Neves; Alexandra T. P. Carvalho; Maria Lourdes Bastos; Vera M. Costa; Félix Carvalho, 2020. Archives of Toxicology. 2020. . 10.1007/s00204-020-02718-1 . Archives of Toxicology

Soft culture substrates favor stem-like cellular phenotype and facilitate reprogramming of human mesenchymal stem/stromal cells (hMSCs) through mechanotransduction

Neves, Ricardo, 2019. Scientific Reports. 2019. . 10.1038/s41598-019-45352-3 . Scientific Reports

Osmotic modulation of chromatin impacts on efficiency and kinetics of cell fate modulation

Neves, Ricardo, 2018. Scientific Reports. 2018. . 10.1038/s41598-018-25517-2 . Scientific Reports

Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression

Márquez-Jurado, S.; Díaz-Colunga, J.; Das Neves, R.P.; Martinez-Lorente, A.; Almazán, F.; Guantes, R.; Iborra, F.J., 2018. Nature Communications. 1. 9. 2018. . 10.1038/s41467-017-02787-4 . Nature Communications

Validating the RedMIT/GFP-LC3 Mouse Model by Studying Mitophagy in Autosomal Dominant Optic Atrophy Due to the OPA1Q285STOP Mutation

Neves, Ricardo, 2018. Frontiers in Cell and Developmental Biology. 2018. . 10.3389/fcell.2018.00103 . Frontiers in Cell and Developmental Biology

Prolonged intracellular accumulation of light-inducible nanoparticles in leukemia cells allows their remote activation

Boto, C.; Quartin, E.; Cai, Y.; Martín-Lorenzo, A.; Cenador, M.B.G.; Pinto, S.; Gupta, R.; et al, 2017. Nature Communications. 8. 2017. . 10.1038/ncomms15204 . Nature Communications

Flexible nanofilms coated with aligned piezoelectric microfibers preserve the contractility of cardiomyocytes

Gouveia, P.J.; Rosa, S.; Ricotti, L.; Abecasis, B.; Almeida, H.V.; Monteiro, L.; Nunes, J.; et al, 2017. Biomaterials. 213 - 228. 139. 2017. . 10.1016/j.biomaterials.2017.05.048 . Biomaterials

Global variability in gene expression and alternative splicing is modulated by mitochondrial content

Guantes, R.; Rastrojo, A.; Neves, R.; Lima, A.; Aguado, B.; Iborra, F.J., 2015. Genome Research. 633 - 644. 5. 125. 2015. . 10.1101/gr.178426.114 . Genome Research

Boron nitride nanotube-mediated stimulation modulates F/G-actin ratio and mechanical properties of human dermal fibroblasts

Ricotti, L.; Das Neves, R.P.; Ciofani, G.; Canale, C.; Nitti, S.; Mattoli, V.; Mazzolai, B.; Ferreira, L.; Menciassi, A., 2014. Journal of Nanoparticle Research. 2. 16. 2014. . 10.1007/s11051-014-2247-z . Journal of Nanoparticle Research

Efficient pro-survival/angiogenic miRNA delivery by an MRI-detectable nanomaterial

Gomes, R.S.M.; Neves, R.P.D.; Cochlin, L.; Lima, A.; Carvalho, R.; Korpisalo, P.; Dragneva, G.; et al, 2013. ACS Nano. 3362 - 3372. 4. 7. 2013. . 10.1021/nn400171w . ACS Nano

Mitochondrial variability as a source of extrinsic cellular noise

Johnston, I.G.; Gaal, B.; das Neves, R.P.; Enver, T.; Iborra, F.J.; Jones, N.S., 2012. PLoS Computational Biology. 3. 8. 2012. . 10.1371/journal.pcbi.1002416 . PLoS Computational Biology

Epigenetic engineering to reverse the Parkinson's expression state

Valente, A.X.C.N.; das Neves, R.P.; Oliveira, P.J., 2012. Parkinsonism and Related Disorders. 717 - 721. 6. 18. 2012. . 10.1016/j.parkreldis.2012.04.018 . Parkinsonism and Related Disorders

Nanoparticles for intracellular-targeted drug delivery

Paulo, C.S.O.; Pires Das Neves, R.; Ferreira, L.S., 2011. Nanotechnology. 49. 22. 2011. . 10.1088/0957-4484/22/49/494002 . Nanotechnology

a-MSH regulates intergenic splicing of MC1R and TUBB3 in human melanocytes

Dalziel, M.; Kolesnichenko, M.; Das Neves, R.P.; Iborra, F.; Goding, C.; Furger, A., 2011. Nucleic Acids Research. 2378 - 2392. 6. 39. 2011. . 10.1093/nar/gkq1125 . Nucleic Acids Research

Towards the maturation and characterization of smooth muscle cells derived from human embryonic stem cells

Vazão, H.; das Neves, R.P.; Grãos, M.; Ferreira, L., 2011. PLoS ONE. 3. 6. 2011. . 10.1371/journal.pone.0017771 . PLoS ONE

MicroRNA-210 regulates mitochondrial free radical response to hypoxia and krebs cycle in cancer cells by targeting iron sulfur cluster protein ISCU

Favaro, E.; Ramachandran, A.; McCormick, R.; Gee, H.; Blancher, C.; Crosby, M.; Devlin, C.; et al, 2010. PLoS ONE. 4. 5. 2010. . 10.1371/journal.pone.0010345 . PLoS ONE

Connecting variability in global transcription rate to mitochondrial variability

das Neves, R.P.; Jones, N.S.; Andreu, L.; Gupta, R.; Enver, T.; Iborra, F.J., 2010. PLoS Biology. 12. 8. 2010. . 10.1371/journal.pbio.1000560 . PLoS Biology

Association between active genes occurs at nuclear speckles and is modulated by chromatin environment

Brown, J.M.; Green, J.; Das Neves, R.P.; Neves, R.P.D.; Wallace, H.A.C.; Smith, A.J.H.; Hughes, J.; et al, 2008. Chemtracts. 411 - 413. 10. 21. 2008. . 10.1083/jcb.200803174 . Chemtracts

Repeated administration of d-amphetamine results in a time-dependent and dose-independent sustained increase in urinary excretion of p-hydroxyamphetamine in mice

Carvalho, F.; Soares, M.E.; Fernandes, E.; Remião, F.; Carvalho, M.; Duarte, J.A.; Pires-das-Neves, R.; Pereira, M.D.L.; Bastos, M.D.L., 2007. Journal of Health Science. 371 - 377. 4. 53. 2007. . 10.1248/jhs.53.371 . Journal of Health Science

Effect of Cr(V) on reproductive organ morphology and sperm parameters: An experimental study in mice

Pereira, M.D.L.; Das Neves, R.P.; Oliveira, H.; Santos, T.M.; De Jesus, J.P., 2005. Environmental Health: A Global Access Science Source. 4. 2005. . 10.1186/1476-069X-4-9 . Environmental Health: A Global Access Science Source

Protective activity of hesperidin and lipoic acid against sodium arsenite acute toxicity in mice

Pires Das Neves, R.N.; Carvalho, F.; Carvalho, M.; Fernandes, E.; Soares, E.; Bastos, M.D.L.; Pereira, M.D.L., 2004. Toxicologic Pathology. 527 - 535. 5. 32. 2004. . 10.1080/01926230490502566 . Toxicologic Pathology

Hypericum androsaemum infusion increases tert-butyl hydroperoxide-induced mice hepatotoxicity in vivo

Valentão, P.; Carvalho, M.; Carvalho, F.; Fernandes, E.; Neves, R.P.D.; Pereira, M.L.; Andrade, P.B.; Seabra, R.M.; Bastos, M.L., 2004. Journal of Ethnopharmacology. 345 - 351. 2-3. 94. 2004. . 10.1016/j.jep.2004.06.012 . Journal of Ethnopharmacology

Comparative histological studies on liver of mice exposed to Cr(VI) and Cr(V) compounds

Pires Das Neves, R.; Santos, T.M.; De Lourdes Pereira, M.; Pedrosa De Jesus, J., 2002. Human and Experimental Toxicology. 365 - 369. 7. 21. 2002. . 10.1191/0960327102ht243oa . Human and Experimental Toxicology

Cr(V) involvement in the toxicity pathway of testicular damage

Pereira, M.L.; Santos, T.M.; Pires das Neves, R.; Costa, F.G.; Pedrosa de Jesus, J., 2002. Asian Journal of Andrology. 153 - 155. 2. 4. 2002. . Asian Journal of Andrology

Effect of 3,4-methylenedioxymethamphetamine ("ecstasy") on body temperature and liver antioxidant status in mice: Influence of ambient temperature

Carvalho, M.; Carvalho, F.; Remião, F.; De Lourdes Pereira, M.; Pires-Das-Neves, R.; De Lourdes Bastos, M., 2002. Archives of Toxicology. 166 - 172. 3. 76. 2002. . 10.1007/s00204-002-0324-z . Archives of Toxicology

Chromium(VI) induced alterations in mouse spleen cells: A short-term assay

Das Neves, R.P.; Santos, T.M.; Pereira, M.D.L.; De Jesus, J.P., 2001. Cytobios. 27 - 34. 1 SUPPL.. 413. 2001. . Cytobios


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